Human FFAR1/GPR40 Antibody

Catalog #: MAB11025 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11025-100
MAB11025-SP

Detection of FFAR1/GPR40 in Human Brain Hypothalamus.

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Human FFAR1/GPR40 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FFAR1/GPR40 in ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1048036

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide within aminoacids 1-50 of human FFAR1/GPR40

Accession # O14842

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

paraffin-embedded sections of human hypothalamus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of FFAR1/GPR40 in Human Brain Hypothalamus. FFAR1/GPR40 was detected in immersion fixed paraffin-embedded sections of human brain hypothalamus using Mouse Anti-Human FFAR1/GPR40 Monoclonal Antibody (Catalog # MAB11025) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FFAR1/GPR40

Human Free Fatty Acid Receptor 1 (FFAR-1), also known as GPR40, is a class A G-protein coupled receptor (GPCR) for medium and long chain saturated and unsaturated fatty acids encoded by the FFAR1 gene. FFAR-1 is expressed in pancreas and it plays an important role in glucose homeostasis acting as a nutrient sensor. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1).

Long Name

Free Fatty Acid Receptor 1

Entrez Gene IDs

2864 (Human); 233081 (Mouse); 266607 (Rat)

Alternate Names

FFA1; FFAR1; free fatty acid receptor 1; G protein-coupled receptor 40; GPCR40; GPR40; GPR40FFA1R; G-protein coupled receptor 40

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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