Human FGF-4 Antibody

Catalog # Availability Size / Price Qty
MAB635
MAB635-SP
Product Details
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Human FGF-4 Antibody Summary

Species Reactivity
Human
Specificity
When used in combination with the biotinylated anti-FGF-4 detection antibody (Catalog # BAF235) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) FGF acidic, bFGF acidic, rhFGF basic, bFGF basic, rhFGF-5, rhFGF-6, or rhFGF-7 (KGF).
Source
Monoclonal Mouse IgG1 Clone # 26019
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human FGF-4
Accession # P08620
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Human FGF-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human FGF‑4 Biotinylated Antibody (Catalog # BAF235)

Standard: Recombinant Human FGF-4 Protein (Catalog # 235-F4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-4

FGF-4 is a member of the FGF family of growth factors. FGF-4 is important in embryonic angiogenesis and limb development and is mitogenic for fibroblasts and endothelial cells.

Long Name
Fibroblast Growth Factor 4
Entrez Gene IDs
2249 (Human); 14175 (Mouse); 116499 (Rat)
Alternate Names
FGF4; FGF-4; fibroblast growth factor 4; HBGF-4; HBGF-4Transforming protein KS3; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST-1; HST-1HSTF-1; HSTF1fibroblast growth factor 4 splice isoform; HSTFGF-4; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; KFGF; K-FGF; KS3; oncogene HST

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