Human Follistatin-like 1/FSTL1 Biotinylated Antibody

Catalog #: BAF1694 Datasheet / COA / SDS

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BAF1694

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Human Follistatin-like 1/FSTL1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Follistatin-like 1/FSTL1 in Western blots. In Western blots, approximately 15% cross‑reactivity with recombinant mouse FSTL1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Follistatin-like 1/FSTL1
Met1-Ile308
Accession # Q12841

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Follistatin-like 1/FSTL1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin-like 1/FSTL1

Follistatin-like 1 (FSL1 or FSTL1), also known as FRP (follistatin-related protein), Flik (follistatin-like), and TSC-36 (TGF-beta 1-stimulated clone 36), is a secreted 45‑55 kDa extracellular glycoprotein belonging to the BM-40/SPARC/Osteonectin family (1-3). The human FSTL1 cDNA encodes 308 amino acids (aa), including a 20 aa signal sequence, a cysteine-rich Follistatin (EGF- and kazal-like) domain, two apparently non-functional EF-hand calcium-binding motifs, and a von Willebrand Factor C homology domain (1, 3). Mature human FSTL1 shares 94%, 95%, 98%, and 99% aa identity with mouse, rat, bovine, and equine FSTL1, respectively. FSTL1 was first identified as a TGF-beta-induced protein from a mouse osteoblast cell line (4). It is ubiquitously expressed in early mouse development, but is mainly mesenchymal later in development (5). In humans, FSTL1 is a common rheumatoid arthritis auto-antigen (2). It is reported to be either pro-inflammatory due to promoting inflammatory cytokine secretion, or to prevent autoimmune arthritis by inhibiting matrix metalloproteinase (MMP) and prostaglandin expression (6-9). In muscle and heart, it appears to be protective and promotes endothelial cell functions such as revascularization after ischemia, probably due to promoting expression and activation of the protein kinase AKT1 (10, 11). Cardiac and circulating FSTL1 is generally increased in conditions such as heart failure and acute coronary syndrome (11, 12). FSTL1 also appears to be a tumor suppressor, showing down-regulated expression in many human cancers (4, 14, 15). In vitro, it slows proliferation and MMP-dependent migration, and increases FAS-dependent apoptosis of tumor cell lines (14).

References

Zwijsen, A. et al. (1994) Eur. J. Biochem. 225:937.
Tanaka, M. et al. (1998) Int. Immunol. 10:1305.
Hambrook, H.O. et al. (2004) J. Biol. Chem. 279:11727.
Shibanuma, M. et al. (1993) Eur. J. Biochem. 217:13.
Adams, D. et al. (2007) Gene Expr. Patterns 7:491.
Clutter, S.D. et al. (2009) J. Immunol. 182:234.
Miyamae, T. et al. (2006) J. Immunol. 177:4758.
Kawabata, D. et al. (2004) Arthritis Rheum. 50:660.
Tanaka, M. et al. (2003) Int. Immunol. 15:71.
Ouchi, N. et al. (2008) J. Biol. Chem. 283:32802.
Oshima, Y. et al. (2008) Circulation 117:3099.
Lara-Pezzi, E. et al. (2008) Endocrinology 149:5822.
Widera, C. et al. (2009) Clin. Chem. 55:1794.
Chan, Q.K. et al. (2009) Carcinogenesis 30:114.
Mashimo, J. et al. (1997) Cancer Lett. 113:213.

Entrez Gene IDs

11167 (Human); 14314 (Mouse); 79210 (Rat)

Alternate Names

Flik; FLJ50214; Follistatin-like 1; Follistatin-like protein 1; follistatin-related protein 1; Follistatin-related Protein; FRP; FRPFLJ52277; FSL1; FSTL1; TSC-36