Human FOLR2 Antibody Summary
Gln22-His228
Accession # P14207
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human FOLR2 by Western Blot. Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FOLR2 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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FOLR2 in Human Neutrophils. FOLR2 was detected in immersion fixed human neutrophils using Human FOLR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FOLR2
Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4‑6). It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8‑10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).
- Fowler, B. et al. (2001) Kidney Int. 59:S-221.
- Kelemen, L.E. (2006) Int. J. Cancer 119:243.
- Shen, F. et al. (1994) Biochemistry 33:1209.
- Ratnam, M. et al. (1989) Biochemistry 28:8249.
- Ross, J.F. et al. (1999) Cancer 85:348.
- Reddy, J.A. et al. (1999) Blood 93:3940.
- Ross, J.F. et al. (1994) Cancer 73:2432.
- Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
- van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
- Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
- Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
- Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.
Product Datasheets
Citations for Human FOLR2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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DECREASED LEVELS OF FOLATE RECEPTOR-beta AND REDUCED NUMBERS OF FETAL MACROPHAGES (HOFBAUER CELLS) IN PLACENTAS FROM PREGNANCIES WITH SEVERE PREECLAMPSIA (PE)*
Authors: Zhonghua Tang, Irina A. Buhimschi, Catalin S. Buhimschi, Serkalem Tadesse, Errol Norwitz, Tracy Niven-Fairchild et al.
American Journal of Reproductive Immunology
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Macrophage Folate Receptor-Targeted Antiretroviral Therapy Facilitates Drug Entry, Retention, Antiretroviral Activities and Biodistribution for Reduction of Human Immunodeficiency Virus Infections.
Authors: Puligujja P, McMillan J, Kendrick L et al.
Nanomedicine
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Glucocorticoids enhance CD163 expression in placental Hofbauer cells.
Authors: Tang Z, Niven-Fairchild T, Tadesse S, Norwitz E, Buhimschi C, Buhimschi I, Guller S
Endocrinology, 2012-11-09;154(1):471-82.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Toll-like Receptor-Mediated Responses by Placental Hofbauer Cells (HBCs): A Potential Pro-Inflammatory Role for Fetal M2 Macrophages*
Authors: Omar M. Young, Zhonghua Tang, Tracy Niven-Fairchild, Serkalem Tadesse, Graciela Krikun, Errol R. Norwitz et al.
American Journal of Reproductive Immunology
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