Human FXYD5/Dysadherin Antibody

Catalog #: AF8300 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8300
AF8300-SP

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Human FXYD5/Dysadherin Antibody Summary

Species Reactivity

Human

Specificity

Detects human FXYD5/Dysadherin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FXYD5/Dysadherin
Gln22-Asp133
Accession # Q96DB9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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FXYD5/Dysadherin in Human Stomach. FXYD5/Dysadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human FXYD5/Dysadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8300) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FXYD5/Dysadherin

Human FXYD5 (FXYD domain-containing ion transport regulator 5 and Dysadherin) is a 24 kDa glycoprotein and a member of the FXYD family of single span type I membrane proteins. This group of proteins has been shown to interact with the Na,K-ATPase and to modulate its properties. FXYD5 is structurally different from other family members and has been suggested to play a role in regulating E-cadherin and promoting metastasis. FXYD5 is preferentially expressed in kidney, intestine, spleen, stomach and lung. Human FXYD5 is 30% aa identical to mouse Fxyd5.

Long Name

FXYD Domain-containing Ion Transport Regulator 5

Entrez Gene IDs

53827 (Human); 18301 (Mouse)

Alternate Names

DYSAD; Dysadherin; EF-8; FXYD domain containing ion transport regulator 5; FXYD domain-containing ion transport regulator 5; FXYD5; IWU1; KCT1; keratinocytes associated transmembrane protein 1; OIT2; PRO6241; RIC