Human Gas6 Antibody

Catalog #: MAB8853 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8853-100
MAB8853-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Gas6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Gas6 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 100130

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Gas6
Asp118-Ala678
Accession # NP_000811

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF885).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Gas6 DuoSet ELISA Kit (Catalog # DY885B) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Gas6 ELISA Standard Curve. Recombinant Human Gas6 protein was serially diluted 2-fold and captured by Mouse Anti-Human Gas6 Monoclonal Antibody (Catalog # MAB8853) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF885) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gas6

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF‑like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

References

Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
Stitt, T.N. et al. (1995) Cell 80:661.
Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
Goruppi, S. et al. (1997) FEBS Lett. 415:59.
Sather, S. et al. (2007) Blood 109:1026.
Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
Gallicchio, M. et al. (2005) Blood 105:1970.
Caraux, A. et al. (2006) Nat. Immunol. 7:747.
Wu, Y. et al. (2005) J. Cell Sci. 118:539.
Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.

Long Name

Growth-arrest-specific Protein 6

Entrez Gene IDs

2621 (Human); 14456 (Mouse)

Alternate Names

AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6