Human GDPD5 Antibody

Catalog #: MAB10524 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10524-100
MAB10524-SP

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Detection of GDPD5 in HEK293 Human Cell Line transfected with human GDPD5 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GDPD5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GDPD5 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 506020

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived transfected with GDPD5
Accession # Q8WTR4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

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Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human GDPD5 and eGFP

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GDPD5 in HEK293 Human Cell Line transfected with human GDPD5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human GDPD5 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-Human GDPD5 monoclonal antibody (Catalog # MAB10524) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody staining (MAB003, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDPD5

Glycerophosphodiester phosphodiesterases, such as GDPD5, are involved in glycerol metabolism. GDPD5 is widely expressed in human tissues. The expression levels in kidney and prostate are relatively low. GDPD5 plays a critical role for glycerophosphodiester metabolism in motor neuron differentiation.

Long Name

Glycerophosphodiester Phosphodiesterase Domain Containing 5

Entrez Gene IDs

81544 (Human); 233552 (Mouse)

Alternate Names

EC 3.1; GDE2; GDE2PP1665Glycerophosphodiester phosphodiesterase 2; GDPD5; glycerophosphodiester phosphodiesterase domain containing 5; glycerophosphodiester phosphodiesterase domain-containing protein 5; PP1665