Human GHRHR Antibody

Catalog #: MAB107351 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB107351-100
MAB107351-SP

GHRHR in HEK293 Human Cell Line transfected with GHRHR.

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Human GHRHR Antibody Summary

Species Reactivity

Human

Specificity

Detects human GHRHR in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1037442

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GHRHR synthetic peptide
Accession # Q02643

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of human pituitary

Immunocytochemistry

8-25 µg/mL

Immersion fixed HEK293 human embryonic kidney cell line transfected with GHRHR

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GHRHR in HEK293 Human Cell Line transfected with GHRHR. GHRHR was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GHRHR (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Mouse Anti-Human GHRHR Monoclonal Antibody (Catalog # MAB107351) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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GHRHR in Human Pituitary. GHRHR was detected in immersion fixed paraffin-embedded sections of human pituitary using Mouse Anti-Human GHRHR Monoclonal Antibody (Catalog # MAB107351) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GHRHR

GHRHR is a GPCR that binds growth hormone-releasing hormone that is expressed in the pituitary. Significant amounts of GHRHR are also found in the hypothalamus, kidney, and placenta. GHRH regulates the secretion of growth hormone that controls the metabolism and growth of every tissue through its binding with GHRHR. Malfunction in GHRHR signaling is associated with abnormal growth. GHRHR is a therapeutic target against dwarfism, gigantism, lipodystrophy, and certain cancers.

Long Name

Growth Hormone Releasing Hormone Receptor

Entrez Gene IDs

2692 (Human); 14602 (Mouse); 25321 (Rat)

Alternate Names

GHRFR; GHRH receptor; GHRHR; GRF receptor; GRFR; growth hormone releasing hormone receptor; Growth hormone-releasing factor receptor; growth hormone-releasing hormone receptor; IGHD1B; Little