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Human GM-CSF Antibody

Catalog #: MAB215
25 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB215-SP
MAB215-500
MAB215-100
GMCSF in Human PBMCs.
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Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GM-CSF Antibody Summary

Species Reactivity
Human
Specificity
Detects human GM-CSF in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 3209
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GM-CSF
Ala18-Glu144
Accession # P04141
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GM-CSF (Catalog # 215-GM)
under non-reducing conditions only
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize GM‑CSF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.075-0.45 µg/mL in the presence of 0.1 ng/mL Recombinant Human GM‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry GMCSF antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

GMCSF in Human PBMCs. Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was detected in human peripheral blood mononuclear cells (PBMCs) using Human GM-CSF Monoclonal Antibody (Catalog # MAB215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by GM‑CSF and Neutralization by Human GM‑CSF Antibody. View Larger

Cell Proliferation Induced by GM‑CSF and Neutralization by Human GM‑CSF Antibody. Recombinant Human GM-CSF (Catalog # 215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human GM-CSF Monoclonal Antibody (Catalog # MAB215). The ND50 is typically 0.075-0.45 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GM-CSF

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Long Name
Granulocyte Macrophage Growth Factor
Entrez Gene IDs
1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names
colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; Molgramostim; molgramostin; Sargramostim

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Citations for Human GM-CSF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance
    Authors: Zheng N, Fang J, Xue G et al.
    Cancer cell
  2. Suppressive myeloid cells are expanded by biliary tract cancer-derived cytokines in vitro and associate with aggressive disease
    Authors: MB Ware, MY Zaidi, J Yang, MK Turgeon, A Krasinskas, TA Mace, K Keenan, MR Farren, AN Ruggieri, Y Li, C Zhang, Z Chen, GS Young, O Elnaggar, Z Che, SK Maithel, T Bekaii-Saa, B El-Rayes, GB Lesinski
    Br. J. Cancer, 2020-08-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts
    Authors: Ruihua Ma, Angelica S Martínez-Ramírez, Thomas L Borders, Fanding Gao, Beatriz Sosa-Pineda
    eLife
  4. Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells
    Authors: LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith
    Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4652-4660.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018-08-15;9(1):3267.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases
    Authors: B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg
    Psychoneuroendocrinology, 2018-07-13;9(1):2705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Activation of neutrophils by Chlamydia trachomatis-infected epithelial cells is modulated by the chlamydial plasmid
    Authors: S Lehr, J Vier, G Häcker, S Kirschnek
    Microbes Infect., 2018-03-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Epstein-Barr virus-induced VEGF and GM-CSF drive nasopharyngeal carcinoma metastasis via recruitment and activation of macrophages
    Authors: D Huang, S Song, ZZ Wu, W Wu, X Cui, JN Chen, MS Zeng, S Su
    Cancer Res., 2017-05-08;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome
    Authors: Lauren W. Wood, Nicole I. Cox, Cody A. Phelps, Shao-Chiang Lai, Arjun Poddar, Conover Talbot et al.
    Scientific Reports
  10. Sphingosine 1 Phosphate at the Blood Brain Barrier: Can the Modulation of S1P Receptor 1 Influence the Response of Endothelial Cells and Astrocytes to Inflammatory Stimuli?
    Authors: Spampinato S, Obermeier B, Cotleur A, Love A, Takeshita Y, Sano Y, Kanda T, Ransohoff R
    PLoS ONE, 2015-07-21;10(7):e0133392.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. GM-CSF promotes migration of human monocytes across the blood brain barrier.
    Authors: Vogel D, Kooij G, Heijnen P, Breur M, Peferoen L, van der Valk P, de Vries H, Amor S, Dijkstra C
    Eur J Immunol, 2015-05-03;45(6):1808-19.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis.
    Authors: Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, Iwamoto Y, Quach T, Ali M, Chen J, Rothstein T, Nahrendorf M, Weissleder R, Swirski F
    J Exp Med, 2014-05-12;211(6):1243-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. A positive feedback loop between mesenchymal-like cancer cells and macrophages is essential to breast cancer metastasis.
    Authors: Su S, Liu Q, Chen J, Chen J, Chen F, He C, Huang D, Wu W, Lin L, Huang W, Zhang J, Cui X, Zheng F, Li H, Yao H, Su F, Song E
    Cancer Cell, 2014-05-12;25(5):605-20.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. BCR-ABL-induced deregulation of the IL-33/ST2 pathway in CD34+ progenitors from chronic myeloid leukemia patients.
    Authors: Levescot A, Flamant S, Basbous S, Jacomet F, Feraud O, Anne Bourgeois E, Bonnet M, Giraud C, Roy L, Barra A, Chomel J, Turhan A, Guilhot F, Girard J, Gombert J, Herbelin A
    Cancer Res, 2014-03-27;74(10):2669-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells.
    Authors: Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, Barra C, Comerma L, Chudnovskiy A, Gentile M, Llige D, Cols M, Serrano S, Arostegui J, Juan M, Yague J, Merad M, Fagarasan S, Cerutti A
    Nat Immunol, 2014-02-23;15(4):354-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. GM-CSF enhances tumor invasion by elevated MMP-2, -9, and -26 expression
    Authors: Claudia M. Gutschalk, Archana K. Yanamandra, Nina Linde, Alice Meides, Sofia Depner, Margareta M. Mueller
    Cancer Medicine
  17. HIV-1–Infected Peripheral Blood Mononuclear Cells Enhance Neutrophil Survival and HLA-DR Expression Via Increased Production of GM-CSF: Implications for HIV-1 Infection
    Authors: Jun Fu, Beverly E. Sha, Larry L. Thomas
    JAIDS Journal of Acquired Immune Deficiency Syndromes
  18. The implication of aberrant GM-CSF expression in decidual cells in the pathogenesis of preeclampsia.
    Authors: Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ
    Am. J. Pathol., 2010-09-09;177(5):2472-82.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-Fr, IHC-P, Neutralization
  19. Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
    Authors: Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ
    J. Invest. Dermatol., 2010-02-11;130(6):1685-96.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007-12-01;117(12):3664-3672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006-11-15;119(10):2313-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006-07-01;54(7):2096-108.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Immunodepletion, Neutralization
  23. Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma.
    Authors: Xiao T, Fujita H, Saeki H, Mitsui H, Sugaya M, Tada Y, Kakinuma T, Torii H, Nakamura K, Asahina A, Tamaki K
    Cytokine, 2003-08-01;23(4):126-32.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
    Authors: Hellman C, 107334, Hallden G, Hylander B, Lundahl J
    Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling, 2003-01-01;131(1):75-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
    Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
    Blood, 2002-01-01;99(1):224-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human GM-CSF Antibody

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By Laboratorio Inmuno-Oncología on 11/23/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human
Immunocytochemistry/Immunofluorescence Human GM-CSF Antibody MAB215
Human GM-CSF Antibody
By Anonymous on 05/02/2018
Application: WB Sample Tested: Pancreatic cancer cells Species: Human