Human GPR39 Antibody

Catalog #: MAB3348 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3348
MAB3348-SP

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Detection of GPR39 in NS0 Mouse Cell Line Transfected with Human GPR39 by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR39 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR39. Stains human GPR39-transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 189702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPR39
Met1-Val453
Accession # BAG37409

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GPR39 in NS0 Mouse Cell Line Transfected with Human GPR39 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human GPR39 was stained with Mouse Anti-Human GPR39 Monoclonal Antibody (Catalog # MAB3348, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR39

G Protein-coupled Receptor 39 (GPR39) is a seven-transmembrane glycoprotein and a member of the Ghrelin receptor family. GPR39 is expressed in the stomach, small intestine and areas of the brain including the hypothalamus; its ligand is the Ghrelin-associated peptide, Obestatin. Obestatin decelerates gastric emptying and decreases intestinal contractility in mice, counteracting the effects of Ghrelin. GPR39 has not been shown to be internalized upon ligand binding. Extracellular loops of human GPR39 show 80% amino acid identity with mouse GPR39.

Long Name

G Protein-coupled Receptor 39

Entrez Gene IDs

2863 (Human); 71111 (Mouse); 288995 (Rat)

Alternate Names

G protein-coupled receptor 39; GPR39; G-protein coupled receptor 39; MGC149541