Human GPR81 Antibody

Catalog #: MAB11134 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11134-100
MAB11134-SP

Detection of Human GPR81 by Western Blot.

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All GPR81 Antibodies

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Human GPR81 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HCAR1/GPFR81 peptide in direct Elisa

Source

Monoclonal Mouse IgG₁ Clone # 1048103

Purification

Protein A or G purified from ascites

Immunogen

Synthetic peptide corresponding to the N-term sequences of Human HCAR1/GPR81

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GPR81 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human GPR81 Monoclonal Antibody (Catalog # MAB11134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for GPR81 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR81

Human Hydroxycarboxylic acid receptor 1 (HCAR1), also known as GPR81 and GPR194, is a G-protein coupled receptor (GPCR) thatt acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.

Long Name

G Protein-coupled Receptor 81

Entrez Gene IDs

27198 (Human); 243270 (Mouse); 689936 (Rat)

Alternate Names

FKSG80; G protein-coupled receptor 104; G protein-coupled receptor 81; GPR104; GPR81; G-protein coupled receptor 104; G-protein coupled receptor 81; HCA1; HCAR1; LACR1; lactate receptor 1; TA-GPCR; T-cell activation G protein-coupled receptor