Human HOIP/RNF31 Antibody Summary
Arg970-Lys1072
Accession # Q96EP0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human HOIP/RNF31 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human HOIP/RNF31 Monoclonal Antibody (Catalog # MAB8039) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HOIP/RNF31 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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HOIP/RNF31 in MCF‑7 Human Cell Line. HOIP/RNF31 was detected in immersion fixed MCF-7 human breast cancer cell line, untreated (upper panel) or treated with Proteasome Inhibitor I (Tocris Bioscience, Catalog # 4045, lower panel), using Mouse Anti-Human HOIP/RNF31 Monoclonal Antibody (Catalog # MAB8039) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human HOIP/RNF31 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for HOIP/RNF31 at approximately 144 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human HOIP/RNF31 Monoclonal Antibody (Catalog # MAB8039). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HOIP/RNF31
RNF31 (RING [Really INteresting Gene] Finger Protein 31; also HOIL-1-interacting protein/HOIP, and Zn in-between-RING-finger ubiquitin-associated domain protein/ZIBRA) is a cytoplasmic E3 ubiquitin-protein ligase that is found in breast epithelium and multiple cancer types. Although its predicted MW is 102 kDa, it runs anomalously at 95-135 kDa in SDS-Page. Ubiquitin (Ub) chains are typically thought of as 9 kDa additions to Lys residues of target molecules. The activity associated with Ub addition depends upon the location of the attachment, and the monomeric vs. polymeric nature of the chains. Ub can also be added to N-terminal Met residues by an intracellular complex called LUBAC (Linear Ub chain Assembly Complex). This complex is key to NF kappa B pathway activation. Following exposure of cells to cytokines, LUBAC ubiquitinates NEMO, which subsequently induces IKK beta phosphorylation, I kappa B alpha degradation, and NF kappa B translocation into the nucleus with gene activation. The LUBAC complex contains RNF31, HOIL-1L and sharpin, and it is now known that RNF31 is the catalyst for linear Ub chain formation. Human RNF31 is 1072 amino acids (aa) in length. It contains three consecutive RanBP2-type Zn finger domains (aa 299-438), a utilized phosphoryation site at Ser466, one UBA domain (aa 564-615), and two RING-type Zn finger domains (aa 699-747 and 860-909) with an intervening IBR-type Zn finger region (aa 779-841). There are at least two isoform variants, one that contains a 13 aa substitution for aa 1-164, and another that shows a deletion of aa 73-630 coupled to a deletion of aa 833-841. Human RNF31 shares 87% aa sequence identity with mouse RNF31.
Product Datasheets
Citations for Human HOIP/RNF31 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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RNF31 inhibition sensitizes tumors to bystander killing by innate and adaptive immune cells
Authors: Zhengkui Zhang, Xiangjun Kong, Maarten A. Ligtenberg, Susan E. van Hal-van Veen, Nils L. Visser, Beaunelle de Bruijn et al.
Cell Reports Medicine
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Mechanistic impact of oligomer poisoning by dominant-negative CARD11 variants
Authors: Jacquelyn R. Bedsaul, Neha Shah, Shelby M. Hutcherson, Joel L. Pomerantz
iScience
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A20 and ABIN-1 cooperate in balancing CBM complex-triggered NF-kappa B signaling in activated T cells
Authors: Hongli Yin, Ozge Karayel, Ying-Yin Chao, Thomas Seeholzer, Isabel Hamp, Oliver Plettenburg et al.
Cellular and Molecular Life Sciences
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Taraxasterol acetate targets RNF31 to inhibit RNF31/p53 axis-driven cell proliferation in colorectal cancer
Authors: Chao-Tao Tang, Jing Yang, Zi-De Liu, Youxiang Chen, Chunyan Zeng
Cell Death Discovery
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Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death
Authors: T Douglas, M Saleh
Cell Rep, 2019-12-10;29(11):3652-3663.e5.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ring finger protein 31-mediated atypical ubiquitination stabilizes forkhead box P3 and thereby stimulates regulatory T-cell function
Authors: F Zhu, G Yi, X Liu, F Zhu, A Zhao, A Wang, R Zhu, Z Chen, B Zhao, S Fang, X Yu, R Lin, R Liang, D Li, W Zhao, Z Zhang, W Guo, S Zhang, S Ge, X Fan, G Zhao, B Li
J. Biol. Chem., 2018-11-02;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Western Blot -
SPATA 2 promotes CYLD activity and regulates TNF ‐induced NF ‐ kappa B signaling and cell death
Authors: Lisa Schlicher, Manuela Wissler, Florian Preiss, Prisca Brauns‐Schubert, Celia Jakob, Veronica Dumit et al.
EMBO reports
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Shigella flexneri suppresses NF-kappa B activation by inhibiting linear ubiquitin chain ligation
Authors: Maarten F. de Jong, Zixu Liu, Didi Chen, Neal M. Alto
Nature Microbiology
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