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Human ICAM-3/CD50 Antibody

Catalog #: BBA29
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BBA29-200
Product Details
Citations (1)
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Human ICAM-3/CD50 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM-3/CD50 in Western blots. Does not cross-react with recombinant human (rh) ICAM-1 or rhVCAM-1.
Source
Monoclonal Mouse IgG1 Clone # Cal 3.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human ICAM-3/CD50 Fc Chimera
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ICAM‑3/CD50 Fc Chimera (Catalog # 715-IC)
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood neutrophils
Adhesion Blockade
Bossy, D. et al. (1995) Eur. J. Immunol. 25:459.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM-3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in mice.

References
  1. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Entrez Gene IDs
3385 (Human)
Alternate Names
CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3

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Citation for Human ICAM-3/CD50 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Macrophage recognition of ICAM-3 on apoptotic leukocytes.
    Authors: Moffatt OD, Devitt A, Bell ED
    J. Immunol., 1999-06-01;162(11):6800-10.
    Species: Human
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Adhesion Assay, Direct ELISA

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