Human IFN-kappa Antibody

Catalog #: MAB10247 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10247-SP	Ships in 5-7 Days	25 ug / $149
MAB10247-100	In Stock	100 ug / $369

Save on RUO reagents! Up to 30% off + FREE shipping. See Details.

Bulk Orders

1 Image

All IFN-kappa Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-kappa Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN‑ kappa in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1009712

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IFN‑ kappa
Leu28-Lys207
Accession # Q90W0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Customers also Viewed

Human Galectin-9 Alexa Fluor® 350-conjugated Antibody

Cat # FAB9064U

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IFN‑ kappa Monoclonal Antibody (Catalog # MAB102472).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

View Larger

Human IFN‑ kappa ELISA Standard Curve. Recombinant Human IFN-kappa protein was serially diluted 2-fold and captured by Mouse Anti-Human IFN-kappa Monoclonal Antibody (Catalog # MAB10247) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IFN-kappa Monoclonal Antibody (Catalog # MAB102472) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-kappa

Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.

Long Name

Interferon kappa

Entrez Gene IDs

56832 (Human); 387510 (Mouse)

Alternate Names

IFNK; IFNkappa; IFN-kappa