Human Ig Kappa Light Chain Antibody

Detection of IgKappa in Human Tonsil via seqIF™ staining on COMET™ IgKappa Antibody was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human IgKappa, Monoclonal Antibody (Catalog # MAB100501) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Human Ig Kappa Light Chain by Western Blot. Western blot shows human kappa light chain protein, human lambda light chain protein, human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ig Kappa Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Ig Kappa Light Chain by Simple Western^TM. Simple Western lane view shows lysates of human kappa light chain protein, human lambda light chain protein (negative control), and human serum, loaded at 0.2 mg/mL. A specific band was detected for Ig Kappa Light Chain at approximately 32 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Human Ig Kappa Light Chain ELISA Standard Curve. Human Ig Kappa Light Chain protein was serially diluted 2-fold and captured by Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Detection of Ig Kappa Light Chain in Human Tonsil. Ig Kappa Light Chain was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.