Human Ig Kappa Light Chain Antibody

Detection of Human Ig Kappa Light Chain by Western Blot. Western blot shows human kappa light chain protein, human lambda light chain protein, human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ig Kappa Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Ig Kappa Light Chain by Simple Western^TM. Simple Western lane view shows lysates of human kappa light chain protein, human lambda light chain protein (negative control), and human serum, loaded at 0.2 mg/mL. A specific band was detected for Ig Kappa Light Chain at approximately 32 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Human Ig Kappa Light Chain ELISA Standard Curve. Human Ig Kappa Light Chain protein was serially diluted 2-fold and captured by Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).