Human IGDCC3 Antibody

Catalog #: MAB8559 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8559
MAB8559-SP

Detection of Human IGDCC3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGDCC3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IGDCC3 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 920038

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human IGDCC3
Met1-Gly640
Accession # Q81VU1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IGDCC3 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock-transfected or transfected with human IGDCC3. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGDCC3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of IGDCC3 in HEK293 Human Cell Line Transfected with Human IGDCC3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human IGDCC3 and eGFP was stained with either (A) Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunocytochemistry

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IGDCC3 in HepG2 Human Cell Line. IGDCC3 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human IGDCC3 Monoclonal Antibody (Catalog # MAB8559) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGDCC3

IGDCC3 (Immunoglobulin superfamily DCC subclass member 3), also known as Punc, is 814 aa single-pass type membrane protein and shares 84% aa identity with mouse IGDCC3. It is a member of the neural cell adhesion molecule family and contains two fibronectin type III repeats and four immunoglobulin-like domains. IDGCC3 is high expressed in the brain and has been suggested to play a role in motor coordination. It has also been suggested to play a role in breast cancer.

Long Name

Immunoglobulin Superfamily, DCC Subclass, Member 3

Entrez Gene IDs

9543 (Human); 19289 (Mouse)

Alternate Names

HsT18880; IGDCC3; immunoglobulin superfamily DCC subclass member 3; immunoglobulin superfamily, DCC subclass, member 3; PUNC; Putative neuronal cell adhesion moleculePUNCHsT18880