Human IGFBP-5 Antibody Summary
Glu28-Glu272
Accession # P24593
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Applications
under non-reducing conditions only
Human IGFBP-5 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Human IGFBP-5 Antibody by Western Blot (A) Elisa determination of IGFBP-2 and -5 concentrations in conditioned medium (CM) from wt and TamR MCF-7 cell lines. Data represent mean ± SD (n = 3). Experiments were repeated on three separate occasions. *p < 0.001, **p < 0.0001 (Student's unpaired t-test). (B) Western blot (upper two panels) and Ligand blot (lower panel) identifies IGFBP-2 (upper arrow Ligand blot) and IGFBP-5 (lower panel Ligand blot) in CM from wt and TamR MCF-7 cells. (C) Densitometric analysis of Western blots for IGFBP-2 (upper graph) and IGFBP-5 (lower graph) band intensity. Triplicate lanes per blot were analysed for both wt and TamR conditioned media using Image Lab software. Data are presented as mean +/− SD (n = 3) of Arbitrary Unit (AU) intensity. This experiment was repeated 3 times with similar results in each instance. *p < 0.005 TamR v MCF-7 Students t-test. GraphPad Prism 5.0. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27050076), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGFBP-5
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-5 cDNA encodes a 272 amino acid (aa) residue precursor protein with a putative 20 aa residue signal peptide that is processed to generate the 252 aa residue mature protein that is post-translationally modified by O-glycosylations and serine phosphorylations. IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment.
- Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
- Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Product Datasheets
Citations for Human IGFBP-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Deregulation of IGF-binding proteins -2 and -5 contributes to the development of endocrine resistant breast cancer in vitro
Oncotarget, 2016-05-31;7(22):32129-43.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Insulin-like growth factor binding protein-5 (IGFBP-5) suppresses the tumourigenesis of head and neck squamous cell carcinoma.
Authors: Hung PS, Kao SY, Shih YH, Chiou SH, Liu CJ, Chang KW, Lin SC
J. Pathol., 2008-02-01;214(3):368-76.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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