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Human IL-10 Antibody

Catalog #: MAB217
30 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB217-SP
MAB217-100
MAB217-500
Detection of Human IL‑10 by Western Blot.
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Product Details
Citations (30)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-10 in Western blots. In Western blots, this antibody shows approximately 5% cross-reactivity with recombinant mouse
IL-10.
Source
Monoclonal Mouse IgG2B Clone # 23738
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑10
Ser19-Asn178
Accession # P22301
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
10 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. At 2 µg/mL, this antibody will neutralize >60% of the bioactivity due to 5 ng/mL Recombinant Human IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-10 antibody by Western Blot. View Larger

Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human IL-10 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human IL‑10 by Simple WesternTM. Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL‑10 at approximately 23 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Human IL-10 Antibody. View Larger

Cell Proliferation Induced by IL‑10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217). At 2 µg/mL, this antibody will neutralize >60% Recombinant Human IL-10 induced bioactivity.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Citations for Human IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva
    Authors: Arpita Saha, Hamdi Ben Halima, Abhishek Saini, Juan Gallardo-Gonzalez, Nadia Zine, Clara Viñas et al.
    Molecules
  2. THBS1+ myeloid cells expand in SLD hepatocellular carcinoma and contribute to immunosuppression and unfavorable prognosis through TREM1
    Authors: Giraud, J;Chalopin, D;Ramel, E;Boyer, T;Zouine, A;Derieppe, MA;Larmonier, N;Adotevi, O;Le Bail, B;Blanc, JF;Laurent, C;Chiche, L;Derive, M;Nikolski, M;Saleh, M;
    Cell reports
    Species: Human
    Sample Types: cell culture
    Applications: Neutralization
  3. In vivo single-cell transcriptomics reveal Klebsiella pneumoniae skews lung macrophages to promote infection
    Authors: A Dumigan, O Cappa, B Morris, J Sá Pessoa, R Calderon-G, G Mills, R Lancaster, D Simpson, A Kissenpfen, JA Bengoechea
    Embo Molecular Medicine, 2022-11-07;0(0):e16888.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
    Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
    Nature Communications, 2022-06-03;13(1):3121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model
    Authors: T Fukasawa, A Yoshizaki, S Ebata, A Yoshizaki-, Y Asano, A Enomoto, K Miyagawa, Y Kazoe, K Mawatari, T Kitamori, S Sato
    Elife, 2021-12-02;10(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Severe Leptospirosis Features in the Spleen Indicate Cellular Immunosuppression Similar to That Found in Septic Shock
    Authors: Amaro Nunes Duarte-Neto, Julio Croda, Carla Pagliari, Francisco Garcia Soriano, Antonio Carlos Nicodemo, Maria Irma Seixas Duarte
    Frontiers in Immunology
  7. The therapeutic HIV Env C5/gp41 vaccine candidate Vacc-C5 induces specific T cell regulation in a phase I/II clinical study
    Authors: K Brekke, M Sommerfelt, M Ökvist, AM Dyrhol-Rii, D Kvale
    BMC Infect. Dis, 2017-03-24;17(1):228.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells
    Authors: BD Hock, SA MacPherson, JL McKenzie
    PLoS ONE, 2017-03-03;12(3):e0172858.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages
    Sci Rep, 2016-08-25;6(0):31959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  10. Potential Use of Interleukin-10 Blockade as a Therapeutic Strategy in Human Cutaneous Leishmaniasis
    Authors: Lucio Roberto Castellano, Laurent Argiro, Helia Dessein, Alain Dessein, Marcos Vinícius da Silva, Dalmo Correia et al.
    Journal of Immunology Research
  11. Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
    Authors: Heine G, Drozdenko G, Grun J, Chang H, Radbruch A, Worm M
    Eur J Immunol, 2014-03-11;44(6):1615-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
    Authors: Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, Klavinskis L, Meiser A, Kelleher P, Dickson G, Patterson S
    PLoS ONE, 2014-02-05;9(2):e88327.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  13. TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.
    Authors: Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L
    Nat Commun, 2014-01-01;5(0):3199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Maintenance of Th1 hepatitis C virus (HCV)-specific responses in individuals with acute HCV who achieve sustained virological clearance after treatment
    Authors: Jacqueline K Flynn, Gregory J Dore, Margaret Hellard, Barbara Yeung, William D Rawlinson, Peter A White et al.
    Journal of Gastroenterology and Hepatology
  15. Pro-inflammatory and anti-inflammatory cytokines in human preterm and term cervical ripening
    Authors: Aurelija Dubicke, Emma Fransson, Gabriele Centini, Eva Andersson, Birgitta Byström, Anders Malmström et al.
    Journal of Reproductive Immunology
  16. Tissue and serum immune response in chronic hepatitis C with mild histological lesions
    Authors: A T R-Viso, M I S Duarte, C Pagliari, E R Fernandes, R A Brasil, G Benard et al.
    Memórias do Instituto Oswaldo Cruz
  17. Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1.
    Authors: Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C
    Eur. J. Immunol., 2008-12-01;38(12):3365-75.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  18. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007-04-01;178(7):4184-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses.
    Authors: Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ
    J. Virol., 2007-03-21;81(11):5882-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  21. Induction of host chemotactic response by Encephalitozoon spp.
    Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
    Infect. Immun., 2006-12-18;75(4):1619-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Increased frequency of CD4+ cells expressing CD161 in cancer patients.
    Authors: Iliopoulou EG, Karamouzis MV, Missitzis I, Ardavanis A, Sotiriadou NN, Baxevanis CN, Rigatos G, Papamichail M, Perez SA
    Clin. Cancer Res., 2006-12-01;12(23):6901-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-09-16;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  25. Distinct transcriptional programs activated by interleukin-10 with or without lipopolysaccharide in dendritic cells: induction of the B cell-activating chemokine, CXC chemokine ligand 13.
    Authors: Perrier P, Martinez FO, Locati M, Bianchi G, Nebuloni M, Vago G, Bazzoni F, Sozzani S, Allavena P, Mantovani A
    J. Immunol., 2004-06-01;172(11):7031-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004-06-01;25(10):1652-9.
    Species: Human
    Sample Types: Serum
    Applications: Affinity Chromatography
  27. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004-06-01;112(2):274-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
    Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004-03-18;104(2):453-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. IL-15 and cognate antigen successfully expand de novo-induced human antigen-specific regulatory CD4+ T cells that require antigen-specific activation for suppression.
    Authors: Koenen HJ, Fasse E, Joosten I
    J. Immunol., 2003-12-15;171(12):6431-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  30. Regulatory activity of autocrine IL-10 on dendritic cell functions.
    Authors: Corinti S, la Sala A, Pastore S
    J. Immunol., 2001-04-01;166(7):4312-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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