Human IL-22 Antibody Summary
Ala34-Ile179
Accession # Q9GZX6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑10 Secretion Induced by IL‑22 and Neutralization by Human IL-22 Antibody. Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-22 Monoclonal Antibody (Catalog # MAB7821). The ND50 is typically 1-5 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
- Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
- Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
- Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Product Datasheets
Citations for Human IL-22 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Modulation of lymphocyte-mediated tissue repair by rational design of heterocyclic aryl hydrocarbon receptor agonists
Authors: J Chen, CA Haller, FE Jernigan, SK Koerner, DJ Wong, Y Wang, JE Cheong, R Kosaraju, J Kwan, DD Park, B Thomas, S Bhasin, RC De La Rosa, AM Premji, L Liu, E Park, AC Moss, A Emili, M Bhasin, L Sun, EL Chaikof
Sci Adv, 2020-01-15;6(3):eaay8230.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Interleukin-22 suppresses the growth of A498 renal cell carcinoma cells via regulation of STAT1 pathway.
Authors: Zhang F, Shang D, Zhang Y, Tian Y
PLoS ONE, 2011-05-23;6(5):e20382.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
TH17 cytokines induce human airway smooth muscle cell migration.
Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
Authors: Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB
PLoS ONE, 2010-11-24;5(11):e14108.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
J. Immunol., 2010-03-31;184(9):4880-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-22 Confers EGFR-TKI Resistance in NSCLC via the AKT and ERK Signaling Pathways
Authors: Xiaomeng Wang, Jiali Xu, Jin Chen, Shidai Jin, Jiaqi Yao, Tongfu Yu et al.
Frontiers in Oncology
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MAB7821 was used as the solid phase antibody, BAM1359 was used as the detection antibody. 1259-RN was used as the immunoassay standard. Sensitivity of the assay was around 100 pg/ml.
