Human IL-5 Antibody Summary
Ile20-Ser134
Accession # P05113
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑5 in Human Peripheral Blood Lymphocytes. IL‑5 was detected in immersion fixed human peripheral blood lymphocytes using Mouse Anti-Human IL‑5 Monoclonal Antibody (Catalog # MAB605) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm of PBMCs. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of IL‑5 in PMA and Ca2+ionomycin-treated Human PBMCs by Flow Cytometry. Human PBMCs, untreated (light orange filled histogram) or activated with 50 ng/mL PMA and 500 ng/mL Ca2+ionomycin for 5 hours (dark orange filled histogram), were stained with Human IL-5 Monoclonal Antibody (Catalog # MAB605) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5
Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross‑reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL‑2‑stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).
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- Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
- Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
- Minamitake, Y. et al. (1990) J. Biochem. 107:292.
- McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
- Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
- Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
- Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
- Clutterbuck, E. J. et al. (1989) Blood 73:1504.
- Cameron, L. et al. (2000) J. Immunol. 164:1538.
- Tavernier, J. et al. (1991) Cell 66:1175.
- Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
- Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
- Tavernier, J. et al. (2000) Blood 95:1600.
- Kopf, M. et al. (1996) Immunity 4:15.
- Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
- Denburg, J. A. et al. (1991) Blood 77:1462.
Product Datasheets
Citations for Human IL-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema
Authors: BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan
Biology, 2021-09-18;10(9):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Impaired activation of lesional CD8+ T-cells is associated with enhanced expression of Programmed Death-1 in Indian Post Kala-azar Dermal Leishmaniasis
Authors: S Mukherjee, R Sengupta, D Mukhopadhy, C Braun, S Mitra, S Roy, N Kanti Das, U Chatterjee, E von Stebut, M Chatterjee
Sci Rep, 2019-01-24;9(1):762.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody
Authors: Daniel Sikkema
J. Immunol. Methods, 2016-11-24;0(0):.
Applications: Competitive Binding Assay -
Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
J. Allergy Clin. Immunol., 2007-11-01;120(5):1118-25.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology, 2004-06-01;112(2):274-89.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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