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Human Integrin  beta 1/CD29 Antibody

Catalog #: MAB1778
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1778
MAB1778-SP
Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 4B7R
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Ocular melanoma cell line V+B2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Integrin  beta 1/CD29 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human skin cancer tissue
Immunoprecipitation
Marshall, J.F. et al. (1998) Br. J. Cancer 77:522.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
Immersion fixed MG-63 human osteosarcoma cell line, A549 human lung carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB1778, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Immunocytochemistry Integrin beta 1/CD29 antibody in MG-63 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 1/CD29 in MG‑63 Human Cell Line. Integrin beta 1/CD29 was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Anti-body (Catalog # MAB1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Anti-body (yellow; NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Integrin  beta 1/CD29 in A549 Cells. Integrin  beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB1778) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits (1).

References
  1. Wayner, E.A. and W.G. Carter (1987) J. Cell Biol. 105:1873.
Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

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Citations for Human Integrin  beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. MicroRNA-141 inhibits epithelial-mesenchymal transition, and ovarian cancer cell migration and invasion
    Authors: Qinghua Ye, Lei Lei, Lingyun Shao, Jing Shi, Jun Jia, Xiaowen Tong
    Molecular Medicine Reports
  2. Nanoparticle biointerfacing by platelet membrane cloaking.
    Authors: Hu C, Fang R, Wang K, Luk B, Thamphiwatana S, Dehaini D, Nguyen P, Angsantikul P, Wen C, Kroll A, Carpenter C, Ramesh M, Qu V, Patel S, Zhu J, Shi W, Hofman F, Chen T, Gao W, Zhang K, Chien S, Zhang L
    Nature, 2015-09-16;526(7571):118-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Bacterial genotoxins promote inside-out integrin beta1 activation, formation of focal adhesion complexes and cell spreading.
    Authors: Levi L, Toyooka T, Patarroyo M, Frisan T
    PLoS ONE, 2015-04-13;10(4):e0124119.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease.
    Authors: Skaleric U, Gaspirc B, McCartney-Francis N, Masera A, Wahl SM
    Infect. Immun., 2006-10-02;74(12):7010-3.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004-06-01;112(2):274-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Blockade of monocyte-endothelial trafficking by transduced Tat-superoxide dismutase protein
    Authors: SIN-HYE PARK, MIN JAE SHIN, DAE WON KIM, JINSEU PARK, SOO YOUNG CHOI, YOUNG-HEE KANG
    International Journal of Molecular Medicine
  7. Rapid induction and long-term self-renewal of neural crest-derived ectodermal chondrogenic cells from hPSCs
    Authors: Pei Shen, Lu Chen, Dahe Zhang, Simo Xia, Zhuman Lv, Duohong Zou et al.
    npj Regenerative Medicine
  8. mTOR kinase inhibition reduces tissue factor expression and growth of pancreatic neuroendocrine tumors
    Authors: Clayton S Lewis, Hala Elnakat Elnakat Thomas, Melissa Orr‐Asman, Lisa C Green, Rachel E Boody, Kateryna Matiash et al.
    Journal of Thrombosis and Haemostasis
  9. Extracellular matrix differences in glioblastoma patients with different prognoses.
    Authors: Virga J, Szivos L et al.
    Oncol Lett

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