Home / Integrin beta 1/CD29 / Human Integrin  beta 1/CD29 Antibody

Human Integrin  beta 1/CD29 Antibody

Catalog #: MAB17782
2 Citations Datasheet / COA / SDS
Newer Version Available: MAB17781
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Discontinued Product

MAB17782 has been discontinued and is replaced by MAB17781.

Integrin beta 1/CD29 in HeLa Human Cell Line.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 1/CD29. Reacts with calcium-dependent epitopes on the human Integrin beta 1 subunit. This antibody activates Integrin  beta 1‑dependent binding to extracellular matrix substrates. The use of binding buffer containing 1 mM Ca+2 is recommended.
Source
Monoclonal Mouse IgG1 Clone # P4G11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
U937 human histiocytic lymphoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood mononuclear cells
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Integrin  beta 1/CD29, see our available Western blot detection antibodies
Adhesion Activation
This antibody was shown to activate Integrin beta 1-mediated cell adhesion. Wayner, E.A. et al. (1993) J. Cell Biol. 121:1141. This application was not tested by R&D Systems.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry

Qin, L. et al. (2003) Biol. Reprod. 69:563 and Kemp, B. et al. (2002) Histochem. Cell. Biol. 117:401.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin beta 1/CD29 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 1/CD29 in HeLa Human Cell Line. Integrin beta 1/CD29 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB17782) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

References
  1. Wayner, E.A. et al. (1993) J. Cell Biol. 121:1141.
Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

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Citations for Human Integrin  beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The tetraspanin CD9 modulates epidermal growth factor receptor signaling in cancer cells.
    Authors: Murayama Y, Shinomura Y, Oritani K, Miyagawa J, Yoshida H, Nishida M, Katsube F, Shiraga M, Miyazaki T, Nakamoto T, Tsutsui S, Tamura S, Higashiyama S, Shimomura I, Hayashi N
    J. Cell. Physiol., 2008-07-01;216(1):135-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  2. Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli.
    Authors: Eto D, Jones T, Sundsbak J, Mulvey M
    PLoS Pathog, 2007-07-01;3(7):e100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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