Human Integrin  beta 1/CD29 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB17781P
Detection of Integrin  beta 1/CD29 in A549 human lung carcinoma cell line by Flow Cytometry.
3 Images
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews

Human Integrin  beta 1/CD29 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 1/CD29 in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # P5D2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human skin keratinocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Knockout Validated
Integrin beta 1/CD29 is specifically detected in A549 lung carcinoma parental cell line but is not detectable in Integrin beta 1/CD29 knockout A549 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin  beta 1/CD29 antibody in A549 human lung carcinoma cell line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in A549 human lung carcinoma cell line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Integrin  beta 1/CD29 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated Integrin  beta 1/CD29 Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

Integrin  beta 1/CD29 Specificity is Shown by Flow Cytometry in Knockout Cell Line. Integrin beta 1/CD29 knockout A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the Integrin beta 1/CD29 knockout A549 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.

References
  1. Barkan, D. and A.F. Chambers (2011) Clin. Cancer Res. 17:7219.
  2. Humphries, M.J. (2000) Biochem. Soc. Trans. 28:311.
Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

Product Datasheets

You must select a language.

x

Citations for Human Integrin  beta 1/CD29 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

  1. Ocular instillation of conditioned medium from mesenchymal stem cells is effective for dry eye syndrome by improving corneal barrier function
    Authors: Imaizumi, T;Hayashi, R;Kudo, Y;Li, X;Yamaguchi, K;Shibata, S;Okubo, T;Ishii, T;Honma, Y;Nishida, K;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Adipose Mesenchymal Stem Cell-Derived Exosomes Promote Wound Healing Through the WNT/ beta -catenin Signaling Pathway in Dermal Fibroblasts
    Authors: Cong Li, Yu An, Yu Sun, Fan Yang, Quanchen Xu, Zhiguo Wang
    Stem Cell Reviews and Reports
  3. Expression Profile of Human Renal Mesangial Cells Is Altered by Infection with Pathogenic Puumala Orthohantavirus
    Authors: C Nusshag, L Boegelein, P Schreiber, S Essbauer, A Osberghaus, M Zeier, E Krautkräme
    Viruses, 2022-04-15;14(4):.
    Species: Chinese Hamster, Human, Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A Comparative Study of Gene Expression in Menstrual Blood-Derived Stromal Cells between Endometriosis and Healthy Women
    Authors: SS Sahraei, F Davoodi As, N Kalhor, M Sheykhhasa, H Fazaeli, SS Moud, A Sheikholes
    BioMed Research International, 2022-01-11;2022(0):7053521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. ATM mediated-p53 signaling pathway forms a novel axis for senescence control
    Authors: SY Hwang, MU Kuk, JW Kim, YH Lee, YS Lee, HE Choy, SC Park, JT Park
    Mitochondrion, 2020-09-17;55(0):54-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
    Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
    Stem Cell Res Ther, 2019-11-04;10(1):316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Negative regulation of osteoclast precursor differentiation by CD11b and beta2 integrin-B-cell lymphoma 6 signaling.
    Authors: Park-Min K, Lee E, Moskowitz N, Lim E, Lee S, Lorenzo J, Huang C, Melnick A, Purdue P, Goldring S, Ivashkiv L
    J Bone Miner Res, 2013-01-01;28(1):135-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Delay of migrating leukocytes by the basement membrane deposited by endothelial cells in long-term culture.
    Authors: Burton VJ, Butler LM, McGettrick HM, Stone PC, Jeffery HC, Savage CO, Rainger GE, Nash GB
    Exp. Cell Res., 2010-11-04;317(3):276-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. A systematic methodology for proteome-wide identification of peptides inhibiting the proliferation and migration of endothelial cells.
    Authors: Karagiannis ED, Popel AS
    Proc. Natl. Acad. Sci. U.S.A., 2008-09-09;105(37):13775-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Integrin  beta 1/CD29 PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Integrin  beta 1/CD29 PE-conjugated Antibody and earn rewards!

Have you used Human Integrin  beta 1/CD29 PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review