Human Irisin/FNDC5 Antibody

Catalog #: MAB8880 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8880-SP	Ships in 5-7 Days	25 ug / $149
MAB8880-100	In Stock	100 ug / $429

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All Irisin/FNDC5 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Irisin/FNDC5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Irisin/FNDC5 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 936410

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Irisin/FNDC5
Asp32-Glu143
Accession # Q8NAU1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

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Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB9420).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Irisin/FNDC5

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).

References

Bostrom, P. et al. (2012) Nature 481:463.
Novelle, M.G. et al. (2013) Int. J. Endocrinol. 2013:746281.
Ferrer-Martinez, A. et al. (2002) Dev. Dyn. 224:154.
Teufel, A. et al. (2002) Gene 297:79.
Zhang, W. et al. (2015) Neurosci. Lett. 595:7.
Roca-Rivada, A. et al. (2013) PLoS One 8:e60563.
Vaughan, R.A. et al. (2014) Diabetes Obes. Metab. 16:711.
Jeong Lee, H. et al. (2015) Mol. Endocrinol. 6:873.
Elsen, M. et al. (2014) J. Endocrinol. 222:R25.
Dun, S.L. et al. (2013) Neuroscience 240:155.
Forouzanfar, M. et al. (2015) Cell. Biol. Int. 39:629.
Hashemi, M.S. et al. (2013) Neuroscience 231:296.
Moon, H.S. et al. (2013) Metabolism 62:1131.
Colaianni, G. et al. (2014) Int. J. Endocrinol. 2014:902186.

Long Name

Fibronectin Type III Domain Containing 5

Entrez Gene IDs

252995 (Human); 384061 (Mouse); 260327 (Rat)

Alternate Names

fibronectin type III domain containing 5; fibronectin type III domain-containing protein 5; FNDC5; FRCP2; FRCP2Fibronectin type III repeat-containing protein 2; Irisin