Human ITFG1 Antibody

Catalog #: MAB8900 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8900
MAB8900-SP

Detection of Human ITFG1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ITFG1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ITFG1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 936234

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived human ITFG1
Met1-Ile566
Accession # Q8TB96

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ITFG1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, human brain (cerebellum) tissue, and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB8900) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ITFG1 at approximately 95-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITFG1

Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology. A ubiquitously expressed 98 kDa glycoprotein, ITFG1 contains an N-terminal signal peptide and a C-terminal transmembrane domain flanking twelve potential N-linked glycosylation sites. Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-g, TNF-a and IL-10, while in vivo ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model. Over amino acids (aa) 1-566, human ITFG1 shares 89% and 88% aa identity with mouse and rat ITFG1, respectively.

Long Name

Integrin Alpha FG-GAP Repeat Containing 1

Entrez Gene IDs

81533 (Human); 71927 (Mouse); 171083 (Rat)

Alternate Names

CDA08; integrin alpha FG-GAP repeat containing 1,2310047C21Rik; Integrin-alpha FG-GAP repeat-containing protein 1; ITFG1; Protein TIP; T-cell immunomodulatory protein; TIP; TIPT cell immunomodulatory protein