Human ITIH2 Antibody

Catalog #: MAB101992 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB101992-SP Ships in 5-7 Days25 ug / $149
MAB101992-100 In Stock100 ug / $379

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ITIH2 in SARS-CoV-2 Infected Human Lung.
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Human ITIH2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ITIH2 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2688A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Spodoptera frugiperda Sf 9 (baculovirus)-derived human ITIH2
Ser55-Asp702
Accession # P19823
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

ITIH2 in SARS-CoV-2 Infected Human Lung. ITIH2 was detected in immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung using Rabbit Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101992) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITIH2

The ITIs are a family of plasma protease inhibitors contributing to extracellular matrix stabilization by covalent linkage to hyaluronan.  ITIH2 is a binding protein between hyaluronan and other matrix proteins.  It regulates the localization, synthesis and degradation of hyaluronan.  ITIH molecules are shown to play a role in carcinogenesis and inflammation.  ITIH genes are downregulated in multiple human solid tumors.  This downregulation contributes to initiation and/or progression of malignancy. 

Long Name
Inter-Alpha-Trypsin Inhibitor Heavy Chain 2
Entrez Gene IDs
3698 (Human); 16425 (Mouse)
Alternate Names
H2P; IGHEP2; Inter-Alpha (Globulin) Inhibitor H2; inter-alpha (globulin) inhibitor, H2 polypeptide; Inter-alpha-inhibitor heavy chain 2; Inter-alpha-trypsin inhibitor complex component II; inter-alpha-trypsin inhibitor heavy chain H2; Intin2; ITI Heavy Chain H2; ITIH2; ITI-H2; ITI-HC2; Serum-derived hyaluronan-associated protein; SHAP

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