Human KLF10 Antibody

Catalog #: AF7016 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7016
AF7016-SP

Detection of Human KLF10 by Western Blot.

1 Image

All KLF10 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KLF10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human KLF10 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human KLF12 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human KLF10
Asn31-Ser154
Accession # Q13118

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human KLF10 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and IMR-32 human neuroblastoma cell line and cytoplasmic extracts of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human KLF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7016) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KLF10 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF10

KLF10 (Kruppel-like factor 10; also TIEG1 and EGR-alpha ) is a 55-65 kDa member of the Sp1 C2H2-type zinc-finger protein family. It appears to be very widely expressed, and functions as a transcriptional repressor in a noncovalent complex with JARID1B. KLF10 is induced by TGF-beta, GDNF and estrogen, and inhibits transcription of genes such as BI-1, OPG and SMAD7. By suppressing SMAD7, it promotes TGF-beta signaling. Human KLF10 is 480 amino acids (aa) in length and contains three C2H2-type zinc-finger motifs (aa 369-451). There is one potential alternate splice form that shows a deletion of aa 2-11. Over aa 31-154, human KLF10 shares 79% aa identity with mouse KLF10/mGIF.

Long Name

Kruppel-Like Factor 10

Entrez Gene IDs

7071 (Human); 21847 (Mouse); 81813 (Rat)

Alternate Names

EGRA; EGR-alpha; KLF10; Krueppel-like factor 10; Kruppel-like factor 10; TGFB inducible early growth response; TGFB-inducible early growth response protein 1; TIEG1; TIEG-1; TIEG1TIEG; Transforming growth factor-beta-inducible early growth response protein 1