Human Lamin B1 Antibody Summary
Met471-Ser582
Accession # P20700
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Lamin B1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Lamin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8525) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lamin B1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Lamin B1 in HeLa Human Cell Line. Lamin B1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Lamin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8525) at 1:150 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclear membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Lamin B1 in Human Liver. Lamin B1 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Lamin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8525) at 1:15 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Western Blot Shows Human Lamin B1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Lamin B1 knockout HeLa cell line (KO). PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Lamin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8525) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lamin B1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Lamin B1
Lamins are components of the nuclear
lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear
membrane, which is thought to provide a framework for the nuclear
envelope and may also interact with chromatin. Lamin B1 is a heavily phosphorylated protein, with a calculated molecular weight of 66kDa.
Product Datasheets
Product Specific Notices
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.FAQs
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