Human Lgr6 Antibody

Catalog #: MAB84581 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB84581-100
MAB84581-SP

Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lgr6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lgr6 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 918726

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Lgr6
Met1-Val967
Accession # Q9HBX8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

In a functional flow cytometry test, biotinylated recombinant human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr6. Binding is completely blocked by 1 μg/mL of Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581).

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Lgr6 in HEK293 Human Cell Line Transfected with Human Lgr6 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Lgr6 and eGFP was stained with either (A) Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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Lgr6 in HEK293 Human Cell Line. Lgr6 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with Lgr6 using Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Blockade of Receptor-ligand Interaction

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R-Spondin 4 Binding to Lgr6-transfected HEK293 Human Cell Line is Blocked by Human Lgr6 Antibody. In a functional flow cytometry test, biotinylated recombinant human R-Spondin 4 (100 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with human Lgr6 (dark orange filled histogram). Binding is completely blocked (light orange filled histogram) by 1 µg/mL of Mouse Anti-Human Lgr6 Monoclonal Antibody (Catalog # MAB84581). Mouse IgG2A Isotype Control (Catalog # MAB003) at 1 µg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr6

Lgr6 is a glycoprotein hormone receptor phosphorylated upon DNA damage, probably by ATM or ATR. It is a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. Lgr6 is a high affinity receptor for R-Spondins 1-3 and potentially functions as a tumor suppressor despite its positive effect on Wnt/ beta -Catenin signaling.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 6

Entrez Gene IDs

59352 (Human); 329252 (Mouse); 498233 (Rat)

Alternate Names

FLJ14471; gonadotropin receptor; GPCR; leucine-rich repeat containing G protein-coupled receptor 6; leucine-rich repeat-containing G protein-coupled receptor 6; leucine-rich repeat-containing G-protein coupled receptor 6; Lgr6; VTS20631