Human LRP-1B Antibody Summary
Leu21-Glu244
Accession # Q9NZR2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human LRP‑1B by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 1 µg/mL of Human LRP-1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6379) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRP-1B
LRP-1B (Low density lipoprotein-related protein #1B; also LRP-DIT) is a 600-620 kDa member of the LDLR family of proteins. It is expressed on vascular smooth muscle cells and likely neurons, and binds multiple molecules, including uPA, PAI-1, PDGFR beta uPAR, and Pseudomonas exotoxin. It is considered a tumor suppressor, and appears to block the generation of plasmin. Mature human LRP-1B is a 4579 amino acid (aa) type I transmembrane (TM) glycoprotein. It contains a 4424 aa extracellular domain (ECD) (aa 21-4444) plus a 132 aa cytoplasmic region (aa 4468-4599). In the ECD, there are 32 LDLR class A repeats, 14 EGF-like repeats, and 36 LDLR class B repeats that show an interspersing pattern. In the cytoplasmic region, one NLS (aa 4468-4474) and two endocytosis signal motifs (aa 4492-4562) are found. LRP-B1 is cleaved in the Golgi by furin after Arg3957 to create a noncovalent heterodimer consisting of a 515 kDa ECD, and an 85-95 kDa TM segment. Either uncleaved LRP-1B, or the dissociated heterodimer can undergo additional processing, leading to cleavage of the 90 kDa TM segment after Lys4435. This creates a 21 kDa TM C-terminal fragment that can undergo further gamma -secretase processing to generate a cytoplasmic, 18 kDa peptide that translocates to the nucleus. Over aa 21-244, human LRP-1B shares 81% aa identity with mouse LRP-1B.
Product Datasheets
Citation for Human LRP-1B Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Inactivating Celsr2 promotes motor axon fasciculation and regeneration in mouse and human
Authors: Quan Wen, Huandi Weng, Tao Liu, Lingtai Yu, Tianyun Zhao, Jingwen Qin et al.
Brain
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