Human LRRC3 Antibody

Catalog #: MAB5039 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5039-SP	Ships in 5-7 Days	25 ug / $149
MAB5039-100	In Stock	100 ug / $399

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LRRC3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LRRC3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 532820

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LRRC3
Cys33-Asp204
Accession # Q9BY71

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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LRRC3 in Human Brain. LRRC3 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human LRRC3 Monoclonal Antibody (Catalog # MAB5039) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC3

LRRC3 (Leu-rich repeat/LRR-containing protein 3) is a 25 kDa (predicted) member of the Leu-rich repeat protein superfamily. Little is known about the protein except for the fact that it shows widespread expression. Human LRRC3 is a protein that is 225 amino acids (aa) in length. It contains three LRRs between aa 63‑86, aa 87‑110 and aa 112‑135. There is one potential splice variant that shows a deletion of aa 129‑248. Over aa 33‑204, human LRRC3 is 83% and 81% aa identical to mouse and canine LRRC3, respectively.

Long Name

Leucine-rich Repeat Containing 3

Entrez Gene IDs

81543 (Human); 237387 (Mouse); 246773 (Rat)

Alternate Names

C21orf102; chromosome 21 open reading frame 102; leucine rich repeat containing 3; leucine-rich repeat-containing protein 3; LRRC3; LRRC3A