Human mGluR1 Antibody

Catalog #: MAB48361 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB48361-100
MAB48361-SP

Detection of MGLUR1 in HEK293 Human Cell Line Transfected with Human MGLUR1 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human mGluR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human mGluR1a in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 511601

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human mGluR1a
Met1-Ser522
Accession # Q13255

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MGLUR1 in HEK293 Human Cell Line Transfected with Human MGLUR1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human MGLUR1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human MGLUR1 Monoclonal Antibody (Catalog # MAB48361) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR1

Metabotropic glutamate receptor 1 (mGluR1 alpha ) is a 130 kDa, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. On neurons, mGluR1 is postsynaptic, associates with G_q-like proteins, mobilizes intracellular Ca⁺⁺, and influences ion channel activity. Mature mGluR1 is 1176 amino acids (aa) in length and contains a 574 aa N-terminal extracellular domain (ECD) (aa 19‑592). The ECD binds glutamate and forms either a covalent homodimer, or heterodimer with CaSR. There is one alternative splice form for human mGluR1 that shows a 20 aa substitution for the C-terminal 308 amino acids. Over aa 33‑522, human mGluR1 shares more that 98% aa identity with mouse, rat and canine mGluR1.

Long Name

Metabotropic Glutamate Receptor 1

Entrez Gene IDs

2911 (Human); 14816 (Mouse); 24414 (Rat)

Alternate Names

glutamate receptor, metabotropic 1; GPRC1A; GRM1; GRM1A; metabotropic glutamate receptor 1; mGluR1; MGLUR1A; MGLUR1GPRC1AmGlu1