Human mGluR3 Antibody

Catalog #: MAB46681 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB46681-100
MAB46681-SP

Detection of mGluR3 in HEK293 Human Cell Line Transfected with Human mGluR3 and eGFP by Flow Cytometry.

1 Image

All mGluR3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human mGluR3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human mGluR3 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 530510

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human mGluR3
Asp25-Ser507
Accession # Q14832

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of mGluR3 in HEK293 Human Cell Line Transfected with Human mGluR3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human mGluR3 and eGFP was stained with (A) Mouse Anti-Human mGluR3 Monoclonal Antibody (Catalog # MAB46681) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR3

Metabotropic glutamate receptor 3 (mGluR3) is a 90-100 kDa, 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. It is a presynaptic receptor expressed on both neurons and glia, whose activation reduces adenylate cyclase activity. Mature human mGluR3 is 857 amino acids in length and contains a 554 amino acid (aa) N-terminal extracellular domain (ECD) (aa 23-576). The ECD binds glutamate and forms homodimers. There is one alternative splice form that is soluble, 515 aa in length and shows a 96 aa substitution for aa 442-879. Over aa 25-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3 and 67% aa sequence identity with hGluR2.

Long Name

Metabotropic Glutamate Receptor 3

Entrez Gene IDs

2913 (Human); 108069 (Mouse); 24416 (Rat)

Alternate Names

GLUR3; glutamate receptor, metabotropic 3; GPRC1C; GPRC1Cglutamate metabotropic receptor 3; GRM3; mGlu3; mGluR3; MGLUR3metabotropic glutamate receptor 3