Human MIG2 Antibody

Catalog #: MAB6984 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6984
MAB6984-SP

Detection of Human MIG2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MIG2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MIG2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MIG, rhFERMT1 (aa 514‑677), rhMIG2/FERMT2 (aa 514‑680), or rhFERMT3 (498‑667) is observed.

Source

Monoclonal Mouse IgG_2B Clone # 696906

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MIG2
Gln321-Leu444
Accession # Q96AC1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MIG2 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MIG2 Monoclonal Antibody (Catalog # MAB6984) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MIG2 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIG2

MIG2 (mitogen-inducible gene-2), also known as FERMT2 or Kindlin-2, is an approximately 80 kDa, 680 amino acid (aa), ubiquitously expressed member of the Fermitin/Kindlin family of cytoplasmic adaptor proteins. MIG2 is essential for beta 1 and beta 3 integrin activity due to its interactions with regulatory molecules such as talin and ILK. MIG2 contains two FERM domains (aa 285-326 and aa 524-573) and one pleckstrin homology domain (aa 373-478). Within the region used as an immunogen, human MIG2 shares 98% aa identity with mouse and rat MIG2. Two isoforms (687 aa and 633 aa) have a 7 aa insert after aa 534. The shorter isoform also has a 2 aa substitution for the C-terminal 57 aa of the 680 aa form.

Long Name

Mitogen Inducible Gene 2

Entrez Gene IDs

10979 (Human); 218952 (Mouse); 289992 (Rat)

Alternate Names

fermitin family homolog 2 (Drosophila); fermitin family homolog 2; fermitin family member 2; FERMT2; KIND2; KIND2DKFZp686G11125; kindlin 2; Kindlin-2; MIG2; mig-2; MIG2FLJ34213; mitogen inducible gene 2 protein; Mitogen-inducible gene 2 protein; PH domain-containing family C member 1; pleckstrin homology domain containing, family C (with FERM domain) member 1; pleckstrin homology domain containing, family C member 1; Pleckstrin homology domain-containing family C member 1; PLEKHC1; PLEKHC1FLJ44462; UNC112; UNC112B