Human/Mouse Glypican 5 Antibody

Name: Human/Mouse Glypican 5 Antibody
Brand: R&D Systems
SKU: MAB2607
Rating: 4 (1 reviews)

Catalog #: MAB2607
4 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2607-SP	Ships in 5-7 Days	25 ug / $149
MAB2607	In Stock	100 ug / $439

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Product Details

Citations (4)

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Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Glypican 5 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse Glypican 5 is observed and no cross‑reactivity with recombinant human Glypican 2, 3 or 6 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 297716

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glypican 5
Glu25-Thr554
Accession # P78333

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

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Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Glypican 5 (Catalog # 2607-G5)
Recombinant Mouse Glypican 5 (Catalog # 2689-G5)

Flow Cytometry

2.5 µg/10⁶ cells

H9 human cutaneous T lymphoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 5

Glypican 5 (GPC5) belongs to the Glypican family of heparan sulfate proteoglycans that are linked to the cell surface through a glycosyl-phosphatidylinositol anchor. Glypican 5 is expressed primarily in embryonic neurons and mesenchyme.

Entrez Gene IDs

2262 (Human); 103978 (Mouse)

Alternate Names

bA93M14.1; Glypican 5; glypican proteoglycan 5; glypican-5; GPC5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Glypican 5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT
Authors: M Takeuchi, K Takeuchi, T Takai, R Yamaguchi, T Furukawa, KI Akagi, JK Takeuchi
PLoS ONE, 2021-02-19;16(2):e0226538.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Heparan sulfate proteoglycans containing a glypican 5 core and 2-O-sulfo-iduronic acid function as Sonic Hedgehog co-receptors to promote proliferation.
Authors: Witt, Rochelle, Hecht, Marie-Ly, Pazyra-Murphy, Maria F, Cohen, Samuel M, Noti, Christia, van Kuppevelt, Toin H, Fuller, Maria, Chan, Jennifer, Hopwood, John J, Seeberger, Peter H, Segal, Rosalind
J Biol Chem, 2013-07-18;288(36):26275-88.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Role for amplification and expression of glypican-5 in rhabdomyosarcoma.
Authors: Williamson D, Selfe J, Lu YJ, Pritchard-Jones K, Murai K, Jones P, Workman P, Shipley J
Cancer Res., 2007-01-01;67(1):57-65.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Glypican-5 stimulates rhabdomyosarcoma cell proliferation by activating Hedgehog signaling
Authors: Fuchuan Li, Wen Shi, Mariana Capurro, Jorge Filmus
Journal of Cell Biology