Human/Mouse MEF2C Antibody

Catalog #: AF6786
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6786
AF6786-SP

Detection of Human MEF2C by Western Blot.

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All MEF2C Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse MEF2C Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human MEF2C in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MEF2C
Ala135-Lys239
Accession # Q06413

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MEF2C by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear (Nuc) extracts. PVDF Membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse MEF2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6786) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for MEF2C at approximately 52-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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MEF2C in Human Brain. MEF2C was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human/ Mouse MEF2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6786) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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MEF2C in Mouse Brain. MEF2C was detected in perfusion fixed frozen sections of mouse brain (cortex) using Sheep Anti-Human/Mouse MEF2C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6786) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal nuclei. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEF2C

MEF2C (Myocyte enhancer factor-2C) is a transcriptional activator that is a member of the MEF2 subfamily, MADS (MCM1, Agamous, Deficiens, Serum response factor) gene family of proteins. Although its predicted MW is 51 kDa, it runs anomalously at 57 kDa in SDS-PAGE. It is expressed in B cells, plus postmitotic neurons and skeletal muscle cells that are undergoing differentiation. Human MEF2C is 473 amino acids (aa) in length. It contains a MADS box for dimerization (aa 3-57), a DNA binding domain (aa 58-86), a beta-domain that enhances transcription (aa 271-278), a gamma-domain that, when phosphorylated, promotes transcriptional repression (aa 390-399), and seven acetylation sites plus multiple Ser/Thr phosphorylation sites. Sumoylation occurs on Lys391, and proteolytic cleavage of MEF2C occurs in neurons between Asp432Gly433. Multiple splice forms exist. Either individually or in combination, there may be a deletion of aa 271-278, 368-399 or 87-134, a 46 aa substitution for aa 107-134 and 87-134, and an alternative start site 35 aa upstream of the standard site. Over aa 135-239, human MEF2C shares 96% aa identity with mouse MEF2C.

Long Name

Myocyte-specific Enhancer Factor 2C

Entrez Gene IDs

4208 (Human); 17260 (Mouse); 499497 (Rat)

Alternate Names

DEL5q14.3; MEF2C; myocyte enhancer factor 2C; myocyte-specific enhancer factor 2C; polypeptide C

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human/Mouse MEF2C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A TBX5-dependent compartment boundary patterns the cardiac interventricular septum
Authors: Kathiriya, IS;Dominguez, MH;Rao, KS;Muncie-Vasic, JM;Patrick Devine, W;Hu, KM;Hota, SK;Garay, BI;Quintero, D;Goyal, P;Matthews, MN;Thomas, R;Sukonnik, T;Miguel-Perez, D;Winchester, S;Brower, EF;Forjaz, A;Wu, PH;Wirtz, D;Kiemen, AL;Bruneau, BG;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Brahma safeguards canalization of cardiac mesoderm differentiation
Authors: SK Hota, KS Rao, AP Blair, A Khalilimey, KM Hu, R Thomas, K So, V Kameswaran, J Xu, BJ Polacco, RV Desai, N Chatterjee, A Hsu, JM Muncie, AM Blotnick, SAB Winchester, LS Weinberger, R Hüttenhain, IS Kathiriya, NJ Krogan, JJ Saucerman, BG Bruneau
Nature, 2022-01-26;602(7895):129-134.
Species: Mouse
Sample Types: Embryos
Applications: IHC