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Human/Mouse/Rat CART Antibody

Catalog #: AF163
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF163
AF163-SP
Detection of CART in Human Brain Hypothalamus Tissue.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat CART Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human CART in direct ELISAs and Western blots. Also recognizes a truncated recombinant human CART containing amino acids 69‑116.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CART
Gln28-Leu116
Accession # Q16568
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CART
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human brain (Hypothalamus) tissue and perfusion fixed frozen sections of mouse brain tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CART antibody in Human Brain Hippocampus Tissue by Immunohistochemistry (IHC-P). View Larger

Detection of CART in Human Brain Hypothalamus Tissue. CART was detected in immersion fixed paraffin-embedded sections of human brain (Hypothalamus) tissue using Goat Anti-Human/Mouse/Rat CART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF163) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry CART antibody in Mouse Brain Tissue by Immunohistochemistry (IHC-Fr). View Larger

CART in Mouse Brain Tissue. CART was detected in perfusion fixed frozen sections of mouse brain tissue using Goat Anti-Human/Mouse/Rat CART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF163) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. Staining was performed using our Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CART

CART, also known as cocaine and amphetamine-regulated transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations. In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment. There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region. There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist. These include a 5K, 48 aa peptide (CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89). Human CART is 98% aa identical to both moyuse and rat CART (1‑89). Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions. In addition, anatomical location is associated with differing lengths of CART. For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

References
  1. Douglass, J. & S. Daoud (1996) Gene 169:241.
  2. Douglass, J. et al. (1995) J. Neurosci. 15:247.
  3. Adams, L.D. et al. (1999) Brain Res. 848:137.
  4. Thim, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2722.
  5. Bannon, A.W. et al. (2001) J. Pharmacol. Exp. Ther. 299:1021.
  6. Kuhar, M.J. & L.L. Yoho (1999) Synapse 33:163.
  7. Thim, L. et al. (1998) FEBS Lett. 428:263.
Long Name
Cocaine- and Amphetamine-regulated Transcript Protein
Entrez Gene IDs
9607 (Human)
Alternate Names
CART prepropeptide; CART; CARTcocaine and amphetamine regulated transcript; CARTPT; cocaine- and amphetamine-regulated transcript protein

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Citations for Human/Mouse/Rat CART Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function
    Authors: Kobori, N;Moore, AN;Redell, JB;Dash, PK;
    Journal of neuroinflammation
  2. MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
    Authors: Cortes-Campos C, Elizondo R, Carril C, Martinez F, Boric K, Nualart F, Garcia-Robles M
    PLoS ONE, 2013-04-26;8(4):e62532.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  3. Edinger-Westphal peptidergic neurons enable maternal preparatory nesting
    Authors: Topilko T, Diaz SL, Pacheco CM et al.
    Neuron
  4. Levels of Cocaine- and Amphetamine-Regulated Transcript in Vagal Afferents in the Mouse Are Unaltered in Response to Metabolic Challenges
    Authors: Laurent Gautron
    eNeuro, 2016-10-05;3(5):.
  5. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART)
    Authors: Ye Long, Andrea S. Bordt, Weiley S. Liu, Elizabeth P. Davis, Stephen J. Lee, Luke Tseng et al.
    Peptides
  6. Increased Hypothalamic Projections to the Lateral Hypothalamus and Responses to Leptin in Rat Neonates From High Fat Fed Mothers
    Authors: Lyla Kelley, Silvanna Verlezza, Hong Long, Mary Loka, Claire-Dominique Walker
    Frontiers in Neuroscience

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