Human/Mouse/Rat JNK1 Antibody

Catalog #: MAB1776
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1776
MAB1776-SP

Detection of human JNK1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat JNK1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat JNK1, expressed predominantly as p46 JNK (isoforms 2 and/or 3, both 384 aa), with lesser amounts of p54 JNK (isoforms 1 and/or 4, both 427 aa), in Western blots. Does not detect recombinant JNK2 or JNK3.

Source

Monoclonal Mouse IgG₁ Clone # 228611

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human JNK1 isoform 2
Met1-Gln384
Accession # NP_002741

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of human JNK1 by Western Blot. Western blot shows recombinant human JNK1, JNK2, and JNK3 (2 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB1776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Western Blot

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Detection of Human, Mouse, and Rat JNK1 by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB1776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for JNK1 were detected at approximately 54 kDa and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JNK1

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Long Name

C-Jun N-terminal Kinase 1

Entrez Gene IDs

5599 (Human); 26420 (Mouse); 116554 (Rat)

Alternate Names

c-Jun N-terminal kinase 1; EC 2.7.11; EC 2.7.11.24; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1; JNK1JNK1A2; JNK-46; JUN N-terminal kinase; MAP kinase 8; MAPK 8; MAPK8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8; PRKM8; PRKM8JNK; protein kinase JNK1; SAPK1; SAPK1JNK21B1/2; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human/Mouse/Rat JNK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1