Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 334708
View all Sortilin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Sortilin
Ser78-Asn755
Accession # Q99523

Specificity

Detects human, mouse, and rat Sortilin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat Sortilin Antibody

Detection of Human Sortilin antibody by Western Blot.

Detection of Human Sortilin by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 95-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Sortilin antibody by Simple WesternTM.

Detection of Human Sortilin by Simple WesternTM.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Sortilin at approximately 87 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse and Rat Sortilin antibody by Western Blot.

Detection of Mouse and Rat Sortilin by Western Blot.

Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Human Sortilin Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Sortilin Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Sortilin knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Sortilin Monoclonal Antibody (Catalog # MAB3154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Sortilin at approximately 100 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat Sortilin Antibody

Application
Recommended Usage

Knockout Validated

Sortilin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Sortilin knockout HeLa cell line.

Simple Western

10 µg/mL
Sample: Human brain (cerebellum) tissue

Western Blot

0.25-1 µg/mL
Sample: Human brain (cerebellum) tissue, human brain (hippocampus) tissue, mouse brain (total) tissue, and rat brain (total) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sortilin

Sortilin, also known as neurotensin receptor 3, is a 100 kDa transmembrane receptor that is localized both in the endosomal compartment and on plasma membranes. It contains an extracellular/lumenal Vps10p (vacuolar protein-sorting 10 protein) domain and is one of five known members of the mammalian Vps10p-D receptor family. A Sortilin propeptide, cleaved by a furin, blocks premature ligand binding to uncleaved Sortilin and functions as a chaperone to facilitate trafficking in the Golgi compartments. Sortilin binds multiple ligands and has diverse functions. Mature human Sortilin extracellular domain shares 92% and 91% amino acid sequence identity with that of mouse and rat Sortilin, respectively.

Alternate Names

Gp95, Ntr3, SORT1

Entrez Gene IDs

6272 (Human); 20661 (Mouse)

Gene Symbol

SORT1

UniProt

Q99523

Additional Sortilin Products

Product Documents for Human/Mouse/Rat Sortilin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Sortilin Antibody

For research use only

Citations for Human/Mouse/Rat Sortilin Antibody

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