Human/Mouse/Rat Transgelin/TAGLN Antibody

Catalog #: MAB7886 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7886
MAB7886-SP

Detection of Human, Mouse, and Rat Transgelin/TAGLN by Western Blot.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Transgelin/TAGLN Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Transgelin/TAGLN in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 859108

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Transgelin/TAGLN
Ala2-Ser201
Accession # Q01995

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat Transgelin/TAGLN by Western Blot. Western blot shows lysates of human aorta tissue, human uterus tissue, mouse uterus tissue, and rat uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Transgelin/ TAGLN Monoclonal Antibody (Catalog # MAB7886) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Transgelin/TAGLN at approximately 16-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transgelin/TAGLN

TAGLN (Transgelin; also 22 kDa Actin-binding protein, WS3-10 and Smooth muscle protein 22 alpha) is a 22-24 kDa cytosolic member of the calponin (calcium-binding and calmodulin-binding troponin T-like protein) family of molecules. It is expressed in both visceral and vascular smooth muscle, fibroblasts, cardiac myocytes, and potentially in breast duct plus prostate epithelium. TAGLN is associated with the actin stress fibers and appears to both suppress MMP-9 production and downmodulate Ca⁺⁺-independent smooth muscle contraction. Human TAGLN is 201 amino acids (aa) in length. It contains one CH/calponin homology domain (aa 24-137), and an actin-binding calponin-like repeat/CLIK (aa 175-200). There is one utilized phosphorylation site at Tyr193, plus two utilized acetylation sites in the N-terminus. Three potential isoform variants are reported, one that shows a alternative start site at Met111, a second that contains a new start site 10 aa upstream of the standard site, and a third that possesses a 58 aa substitution for aa 97-201. Full-length human and mouse TAGLN share 97% aa sequence identity.

Entrez Gene IDs

6876 (Human); 21345 (Mouse); 25123 (Rat)

Alternate Names

DKFZp686P11128; Protein WS3-10; SM22 alpha; SM2222 kDa actin-binding protein; SMCCDKFZp686B01212; Smooth muscle protein 22-alpha; TAGLN; TAGLN1; transgelin variant 2; Transgelin; WS3-10; WS3-10SM22-alpha