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Human MRGX2 Antibody

Catalog #: MAB4727
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4727
MAB4727-SP
Detection of MRGX2 in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MRGX2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MRGX2. Stains human MRGX2 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 477533
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human MRGX2
Met1-Val330
Accession # Q96LB1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µL/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MRGX2 antibody in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP antibody by Flow Cytometry. View Larger

Detection of MRGX2 in HEK293 Human Cell Line Transfected with Human MRGX2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human MRGX2 and eGFP was stained with Mouse Anti-Human MRGX2 Monoclonal Antibody (Catalog # MAB4727) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Intracellular Staining by Flow Cytometry Detection of MRGX2 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MRGX2 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human MRGX2 Monoclonal Antibody (Catalog # MAB4727, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MRGX2

MRGX2 (MAS-related GPR, member X2) is a 330 amino acid (aa) G-protein coupled 7-transmembrane protein that is selectively expressed in small-diameter sensory neurons of dorsal root ganglia. Human MRGX shares 41‑52% aa identity with three other primate MRGX proteins. Binding of ligands such as cortistatin, proadrenomedullin peptides (PAMP-12 and –20) and basic peptides (substance P, neuropeptide Y) to MRGX2 can activate Gq or Gi regulated pathways. MRGX2 is thought to influence nociception and promote adrenal gland catecholamine secretion and IgE-independent mast cell degranulation.

Long Name
Mas-related G Protein-coupled Receptor Member X2
Entrez Gene IDs
117194 (Human); 243978 (Mouse)
Alternate Names
MAS-related GPR, member X2; mas-related G-protein coupled receptor member X2; MRGPRX2; MRGX2; MRGX2G protein-coupled receptor MRGX2

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Citations for Human MRGX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The dual regulation of substance P-mediated inflammation via human synovial mast cells in rheumatoid arthritis
    Authors: Y Okamura, S Mishima, JI Kashiwakur, T Sasaki-Sak, S Toyoshima, K Kuroda, S Saito, Y Tokuhashi, Y Okayama
    Allergol Int, 2017-03-31;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria.
    Authors: Fujisawa D, Kashiwakura J, Kita H, Kikukawa Y, Fujitani Y, Sasaki-Sakamoto T, Kuroda K, Nunomura S, Hayama K, Terui T, Ra C, Okayama Y
    J Allergy Clin Immunol, 2014-06-19;134(3):622-633.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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