Human MSX1 Antibody

Catalog #: MAB5045 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5045
MAB5045-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MSX1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MSX1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MSX2 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 801601

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MSX1
Met1-Thr165
Accession # P28360

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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MSX1 in Human Ovary. MSX1 was detected in immersion fixed paraffin-embedded sections of human ovary using Mouse Anti-Human MSX1 Monoclonal Antibody (Catalog # MAB5045) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSX1

MSX1 (Msh homeobox homology 1) is a member of the muscle segment homoebox gene family. MSX1 is involved in limb-pattern formation, craniofacial development, odontogenesis, and tumor growth inhibition. MSX1 functions as a transcriptional repressor. MSX1 has been shown to interact with the linker histone, H1B, and repress transcription of the MyoD promoter. Chromosomal abnormalities involving MSX1 have been associated with the Wolf-Hirschhorn syndrome characterized by heart defects and mental retardation.

Long Name

Msh Homeobox 1

Entrez Gene IDs

4487 (Human); 17701 (Mouse); 81710 (Rat)

Alternate Names

Homeobox protein Hox-7; homeobox protein MSX-1; HOX7; HOX7homeobox 7; HYD1; HYD1msh homeobox homolog 1 (Drosophila); msh (Drosophila) homeo box homolog 1 (formerly homeo box 7); msh homeo box 1; msh homeobox 1; Msh homeobox 1-like protein; msh homeobox homolog 1; MSX1; OFC5; STHAG1