Human NAIP Antibody

Catalog #: AF829
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF829
AF829-SP

Detection of Human NAIP by Western Blot.

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All NAIP Antibodies

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Citations (3)

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Human NAIP Antibody Summary

Species Reactivity

Human

Specificity

Detects human NAIP expressed in Sf 21 insect cells.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

KLH-coupled human NAIP synthetic peptide
YLSLSSTRPDEGLASIIC
Accession # NP_004527

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NAIP by Western Blot. Western blot shows lysates ofSf21 insect cells before (lane 1) and 48 hours post infection with human NAIP (lane 2). PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human NAIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF829) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for truncated NAIP was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAIP

Neuronal apoptosis inhibitory protein (NAIP; also Baculoviral IAP repeat-containing protein 1) is a 160 kDa member of the inhibitor of apoptosis family of proteins (also known as BIRC proteins). Human NAIP is 1403 amino acids (aa) in length. It contains three distinct regions: an N-terminal cluster of three baculoviral inhibitory repeat (BIR) domains, a central nucleotide binding oligomerization domain (NOD), and a C-terminal leucine-rich repeat (LRR) domain. Human NAIP shares 68% aa identity with mouse NAIP. NAIP is expressed in motor neurons, but not in sensory neurons. It is also expressed in the liver, placenta and to a lesser extent in the spinal cord. NAIP prevents motor neuron apoptosis, and defects in NAIP have been found in individuals with spinal muscular atrophy.

Long Name

Neuronal Apoptosis Inhibitor Protein

Entrez Gene IDs

4671 (Human); 17940 (Mouse); 191568 (Rat)

Alternate Names

baculoviral IAP repeat-containing 1; BIRC1; BIRC1baculoviral IAP repeat-containing protein 1; FLJ18088; FLJ42520; FLJ58811; NAIP; Neuronal apoptosis inhibitory protein; NLR family, apoptosis inhibitory protein; NLR family, BIR domain containing 1; NLRB1; nucleotide-binding oligomerization domain, leucine rich repeat and BIR domaincontaining 1; psi neuronal apoptosis inhibitory protein; psiNAIP

Product Datasheets

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Related Research Areas

Citations for Human NAIP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
Authors: Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
Eur. J. Immunol., 2006-07-01;36(7):1975-84.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Inflammation-associated cell cycle-independent block of apoptosis by survivin in terminally differentiated neutrophils.
Authors: Altznauer F, Martinelli S, Yousefi S, Thurig C, Schmid I, Conway EM, Schoni MH, Vogt P, Mueller C, Fey MF, Zangemeister-Wittke U, Simon HU
J. Exp. Med., 2004-05-17;199(10):1343-54.
Species: Human
Sample Types: Whole Cells
Decreased apoptosome activity with neuronal differentiation sets the threshold for strict IAP regulation of apoptosis
Authors: Kevin M. Wright, Michael W. Linhoff, Patrick Ryan Potts, Mohanish Deshmukh
The Journal of Cell Biology