Human NrCAM Antibody

Catalog #: MAB20341 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB20341
MAB20341-SP

All NrCAM Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NrCAM Antibody Summary

Species Reactivity

Human

Specificity

Detects human NrCAM in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 297909

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NrCAM isoform 3
Leu30-Asn600 (Ala526Pro)
Accession # Q14CA1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human NrCAM Fc Chimera (Catalog # 2034-NR)
under non-reducing conditions only

Human NrCAM Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human NrCAM Isoform 3 Biotinylated Antibody (Catalog # BAF2034)

Standard: Recombinant Human NrCAM Fc Chimera Protein, CF (Catalog # 2034-NR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NrCAM

NgCAM related cell adhesion molecule (NrCAM), also known as Bravo, belongs to the L1 family of cell adhesion molecules, which also include L1, neurofascin and close homolog of L1 (CHL-1) (1). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4 - 5 fibronectin type III-like domains in their extracellular domain. They also shared a conserved cytoplasmic region containing an ankyrin-binding site. L1 family cell adhesion molecules are expressed primarily in the nervous system where they share overlapping functions in controlling axonal growth and guidance (1, 2). NrCam mediates homophilic adhesion as well as heterophilic adhesion with a number of neuronal adhesion molecules including contactin, TAG-1/contactin-2, neurofascin and receptor tyrosine phosphatase beta (RPTP beta ) (3-5). NrCAM has been implicated in the axogenesis of multiple neuronal populations including sensory axons and commissural axons in the spinal cord of chick embryos. It also plays a role in cerebellar granule cell development, and is required in node of Ranvier formation (5, 6).

References

Hortsch, M. (2000) Mol. Cell. Neurosci. 15:1.
Stoeckli, E.T. and L.T. Landmesser (1995) Neruon 14:1165.
Mauro, V.P. et al. (1992) 119:191.
Fustig, M. Sakurai, T. and M. Grumet (1999) Dev. Bio. 209:340.
Sakurai, T. et al. (2001) J. Cell Bio. 154:1259.
Custer, A.W. et al. (2003) J. Neuroscience 23:10032.

Long Name

Neuronal Cell Adhesion Molecule

Entrez Gene IDs

4897 (Human); 319504 (Mouse)

Alternate Names

Bravo; hBravo; KIAA0343Neuronal surface protein Bravo; MGC138845; MGC138846; neuronal cell adhesion molecule; NgCAM-related cell adhesion molecule; ng-CAM-related; NrCAM; nr-CAM