Home / 53BP1 / Human Phospho-53BP1 (S25) Antibody

Human Phospho-53BP1 (S25) Antibody

Catalog #: AF3405
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3405
AF3405-SP
Detection of Human Phospho-53BP1 (S25) by Western Blot.
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Human Phospho-53BP1 (S25) Antibody Summary

Species Reactivity
Human
Specificity
Detects human 53BP1 when phosphorylated at S25.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human 53BP1 S25 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-53BP1 (S25) antibody by Western Blot. View Larger

Detection of Human Phospho-53BP1 (S25) by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line untreated (-) or exposed (+) to 50 J/m2UV-C and allowed 1 hour recovery time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-53BP1 (S25) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3405) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-53BP1 (S25) was detected at approximately 450 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 53BP1

The p53-binding protein 1 (53BP1) localizes to sites of damaged DNA and is phosphorylated by the ATM checkpoint kinase. 53BP1 is required for phosphorylation of other downstream ATM substrates, such as p53 and SMC-1, and is itself a substrate for the ATM kinase. ATM phosphorylates 53BP1 at S25, and this phosphorylation aids in 53BP1 nucleating a DNA damage response protein complex.

Long Name
p53 Binding Protein 1
Entrez Gene IDs
7158 (Human); 27223 (Mouse); 296099 (Rat)
Alternate Names
53BP1; p202; p53-binding protein 1; p53bp1; TDRD30; TP53-Binding Protein 1; TP53BP1; tumor protein 53-binding protein, 1; tumor protein p53 binding protein 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1

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Citation for Human Phospho-53BP1 (S25) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. 53BP1 promotes ATM activity through direct interactions with the MRN complex.
    Authors: Lee JH, Goodarzi AA, Jeggo PA, Paull TT
    EMBO J., 2009-12-10;29(3):574-85.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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