Home / Lck / Human Phospho-Lck (Y394) Antibody

Human Phospho-Lck (Y394) Antibody

Catalog #: MAB7500
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7500
MAB7500-SP
Detection of Human Phospho-Lck (Y394) by Western Blot.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-Lck (Y394) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Lck when phosphorylated at Y394. Due to high sequence homology, this antibody may cross-react with phosphorylated members of the Src family.
Source
Monoclonal Mouse IgG2B Clone # 755103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human Lck Y394 site
Accession # P06239
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-Lck (Y394) antibody by Western Blot. View Larger

Detection of Human Phospho-Lck (Y394) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Lck (Y394) Monoclonal Antibody (Catalog # MAB7500) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Lck (Y394) at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-Lck (Y394) antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho‑Lck (Y394) in Jurkat Human Cell Line. Lck phosphorylated at Y394 was detected in immersion fixed Jurkat human acute T cell leukemia cells treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Lck (Y394) Monoclonal Antibody (Catalog # MAB7500) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lck

Lck (p56lck) is a 56 kDa cytosolic phosphoprotein within the Src family of non-receptor tyrosine kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121), an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). A short (363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4 and CD8 molecules and plays a pivotal role in regulating T cell activation. It can be overexpressed in cancers and functions as an oncogene. Y394 is the major site for autophosphorylation that enhances kinase activity, while Y505 is the major phosphorylation site for negative regulation.

Long Name
Lymphocyte Protein Tyrosine Kinase
Entrez Gene IDs
3932 (Human); 16818 (Mouse); 313050 (Rat)
Alternate Names
EC 2.7.10; EC 2.7.10.2; Hck-3; Lck; Lcktkr; Leukocyte C-terminal Src kinase; LSK; LSTRA; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; p56lck; p56-LCK; pp58lck; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine-protein kinase Lck; YT16

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Citations for Human Phospho-Lck (Y394) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Dual Targeting of Apoptotic and Signaling Pathways in T-Lineage Acute Lymphoblastic Leukemia
    Authors: Caner Saygin, Giorgia Giordano, Kathryn Shimamoto, Bart Eisfelder, Anika Thomas-Toth, Girish Venkataraman et al.
    Clinical Cancer Research
  2. Polo-like kinase 1 regulates immune synapse assembly and cytotoxic T cell function by driving microtubule dynamics
    Authors: Zevolini, F;Onnis, A;Khazen, R;Müller, S;Marotta, G;Valitutti, S;Finetti, F;Baldari, CT;
    Journal of cell science
    Species: Human
    Sample Types: Cell Lysates, Transfected Whole Cells, Whole Cells
    Applications: ICC, Western Blot
  3. Allosteric inhibition of the T cell receptor by a designed membrane ligand
    Authors: Ye, Y;Morita, S;Chang, JJ;Buckley, PM;Wilhelm, KB;DiMaio, D;Groves, JT;Barrera, FN;
    eLife
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer
    Authors: Dey, G;Bharti, R;Braley, C;Alluri, R;Esakov, E;Crean-Tate, K;McCrae, K;Joehlin-Price, A;Rose, PG;Lathia, J;Gong, Z;Reizes, O;
    Journal of ovarian research
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. A short hepatitis C virus NS5A peptide expression by AAV vector modulates human T cell activation and reduces vector immunogenicity
    Authors: W Colon-Mora, A Baer, G Lamture, JT Stapleton, JW Fischer, N Bhattarai
    Gene Therapy, 2021-11-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Pretreatment with LCK inhibitors chemosensitizes cisplatin-resistant endometrioid ovarian tumors
    Authors: KK Crean-Tate, C Braley, G Dey, E Esakov, C Saygin, A Trestan, DJ Silver, SM Turaga, EV Connor, R DeBernardo, CM Michener, PG Rose, J Lathia, O Reizes
    Journal of ovarian research, 2021-04-22;14(1):55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. TAOK3 Regulates Canonical TCR Signaling by Preventing Early SHP-1-Mediated Inactivation of LCK
    Authors: JVS Ormonde, Z Li, C Stegen, J Madrenas
    J. Immunol., 2018-10-29;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Characterization of the effects of immunomodulatory drug fingolimod (FTY720) on human T cell receptor signaling pathways
    Authors: A Baer, W Colon-Mora, N Bhattarai
    Sci Rep, 2018-07-19;8(1):10910.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Nuclear pore complex-mediated modulation of TCR signaling is required for na�ve CD4+ T cell homeostasis
    Authors: J Borlido, S Sakuma, M Raices, F Carrette, R Tinoco, LM Bradley, MA D'Angelo
    Nat. Immunol., 2018-05-07;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Hepatitis C virus infection inhibits a Src-kinase regulatory phosphatase and reduces T cell activation in vivo
    Authors: N Bhattarai, JH McLinden, J Xiang, MM Mathahs, WN Schmidt, TM Kaufman, JT Stapleton
    PLoS Pathog, 2017-02-24;13(2):e1006232.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
    Authors: Clayton K, Haaland M, Douglas-Vail M, Mujib S, Chew G, Ndhlovu L, Ostrowski M
    J Immunol, 2013-12-13;192(2):782-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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