Human Phospho-MLKL (T357) Antibody

Catalog #: MAB9187
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9187-100
MAB9187-SP

Phospho-MLKL (T357) in Human Squamous Cell Carcinoma.

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Citations (2)

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Human Phospho-MLKL (T357) Antibody Summary

Species Reactivity

Human

Specificity

Detects human MLKL when phosphorylated at T357.

Source

Monoclonal Mouse IgG_2A Clone # 954702

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Phosphopeptide containing the human MLKL T357 site
Accession # Q8NB16

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Phospho-MLKL (T357) in Human Squamous Cell Carcinoma. MLKL phosphorylated at T357 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Phospho-MLKL (T357) Monoclonal Antibody (Catalog # MAB9187) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MLKL

Mixed Lineage Kinase Domain-Like (MLKL) is a 471 amino acid member of the protein kinase superfamily, but lacks several amino acid residues necessary for kinase activity. Phosphorylation of MLKL at T357 and S358 by receptor-interacting protein 3 (RIP3) causes trimerization and translocation of MLKL from the cytosol to the plasma membrane, where it mediates TNF-alpha induced necroptosis through membrane degradation and calcium influx. Low expression of MLKL has been found to be correlated with poor prognosis in cervical squamous cell carcinoma, pancreatic cancer, and ovarian cancer. Conversely, high expression of RIPK3 and MLKL in esophageal and colon cancer is associated with poor overall survival, possibly by promoting inflammatory cytokine production, sustaining tumor growth.

Long Name

Mixed Lineage Kinase Domain-like

Entrez Gene IDs

197259 (Human); 74568 (Mouse); 690743 (Rat)

Alternate Names

FLJ34389; mixed lineage kinase domain-like protein; mixed lineage kinase domain-like; MLKL

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human Phospho-MLKL (T357) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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