Human Phospho-RPA2 (T21) Antibody

Catalog #: AF6654 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6654
AF6654-SP

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Detection of Human Phospho‑RPA2 (T21) by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-RPA2 (T21) Antibody Summary

Species Reactivity

Human

Specificity

Detects human RPA2 when phosphorylated at T21.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing the human RPA2 T21 site
Accession # P15927

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and Sodium Azide with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho‑RPA2 (T21) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and U2OS human osteosarcoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour. PVDF Membrane was probed with 0.5 µg/mL of Human Phospho-RPA2 (T21) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6654) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RPA2 (T21) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RPA2

RPA2 (replication protein A 32 kDa subunit; also RFA2 and RPA p34) is a 32 kDa DNA-binding protein that constitutess one of three subunits comprising the PRA heterotrimer complex. In conjunction with 70 kDa RPA1 and 14 kDa RPA3, RPA2 participates in DNA replication, recombination and repair. Human RPA2 is 270 amino acids (aa) in length. It contains a Gly/Ser-rich N-terminus (aa 1-33), a DNA-binding domain (aa 43-171) and a protein-interaction C-terminus (aa 187-270). Phosphorylation of the N-terminus on Ser4/8/23/29/33, plus Thr21, regulates RPA complex interactions with DNA repair and replication complexes. There are multiple splice variants. Three contain N-terminal extensions: one shows an 88 aa insertion after Ser4, another shows a 12 aa substitution for aa 1-4, and a third shows a four aa insertion after Ser4. There is also a deletion of aa 93-98, and a potential truncation after Gln175. Over aa 141-270, human RPA2 shares 83% aa identity with mouse RPA2.

Long Name

Replication Protein A2, 32kDa

Entrez Gene IDs

6118 (Human); 19891 (Mouse); 59102 (Rat)

Alternate Names

REPA2; Replication factor A protein 2; replication protein A 32 kDa subunit; Replication protein A 34 kDa subunit; replication protein A2 (32kD); replication protein A2, 32kDa; RF-A protein 2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34