Human Plasminogen Antibody Summary
Glu20-Asn810
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Neutralization of Plasminogen Activity by Human Plasminogen Antibody. Neutralization of Plasminogen Activity by Human Plasminogen Antibody.](https://resources.rndsystems.com/images/datasheets/antibody/Plasminogen_MAB1939_Block_Neutralize_11271.jpg)
Neutralization of Plasminogen Activity by Human Plasminogen Antibody. The cleavage of Suc-AFK-AMC (100 µM) by Human Plasminogen (1 µg/mL, Catalog # 1939-SE) is measured after preincubation with increasing concentrations of Mouse Anti-Human Plasminogen Monoclonal Antibody (Catalog # MAB1939). The ND50 is typically 1 µg/mL.
![Detection of Plasminogen by Western Blot Detection of Plasminogen by Western Blot](https://resources.rndsystems.com/images/datasheets/mab1939_human-plasminogen-mab-clone-270409-8120255531694.jpg)
Detection of Plasminogen by Western Blot Diminished PLG in psoriasis.(A) A significant reduction in the expression of PLG in PBMC of psoriasis patients was detected by flow cytometry. (B) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with a PLG mAb (red). (C) Lysates from lesional skin of psoriasis patients or normal skin of volunteers were subjected to western blotting for PLG expression. Human PLG was used as a positive control (right panel). Actin - loading control. (D, E) Cryosections from lesional skin of psoriasis patients or normal volunteers were stained with uPA or tPA mAbs (green) for its expression. Original magnification for immunofluorescent staining, ×400. e, epidermis; d, dermis. Dotted lines indicate the border between epidermis and dermis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/21311769), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plasminogen
Plasminogen (PLG) is the precursor of plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation, and tumor invasion (1, 2). Synthesized in the kidney, PLG is found in plasma and many extracellular fluids. Activated by u- or t-plasminogen activator, the single-chain PLG (amino acid residues 20-810) is converted to plasmin, which consists of disulfide bond-linked heavy chain A (residues 20-580) and light chain B (residues 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named as angiostatin, a novel angiogenesis inhibitor (3, 4).
- Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
- Forsgren, M. et al. (1987) FEBS Lett. 213:254.
- O’Reilly, M.S. et al. (1994) Cell 79:315.
- Sim, B.K. et al. (1997) Cancer Res. 57:1329.
Product Datasheets
Citations for Human Plasminogen Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Runx1 promotes scar deposition and inhibits myocardial proliferation and survival during zebrafish heart regeneration
Authors: Jana Koth, Xiaonan Wang, Abigail C. Killen, William T. Stockdale, Helen G. Potts, Andrew Jefferson et al.
Development
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A novel role for plasmin-mediated degradation of opsonizing antibody in the evasion of host immunity by virulent, but not attenuated, Francisella tularensis.
Authors: Crane DD, Warner SL, Bosio CM
J. Immunol., 2009-09-14;183(7):4593-600.
Species: Human
Sample Types: Buffer
Applications: ELISA Development
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