Human Plexin D1 Antibody Summary
Leu47-Ala1271
Accession # Q9Y4D7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Plexin D1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Plexin D1 Monoclonal Antibody (Catalog # MAB41601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Plexin D1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Zebrafish Plexin D1 by Western Blot Efficient shRNA-mediated knockdown of GIPCs and CRISPR/Cas9-mediated knockout of PLXND1 in HUVEC/TERT2 cells.(A–H) Western blots for GIPC1-2, PLXND1, and GAPDH (loading control) from TCLs of stable cells demonstrating the effective decrease of GIPC1-2 and PLXND1 levels. (A–D) TCLs from non-targeting gRNA#1 cells (A, C) and PLXND1 gRNA KO#1 cells (B, D) infected with the indicated shRNAs and under the different SEMA3E treatments. (E–H) TCLs from non-targeting gRNA#2 cells (E, G) and PLXND1 gRNA KO#2 cells (F, H) infected with the indicated shRNAs and under the different SEMA3E treatments. This figure is related to Figure 7 and Figure 7—figure supplement 1. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31050647), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Zebrafish Plexin D1 by Western Blot Efficient shRNA-mediated GIPC (GIPC1, GIPC2, and GIPC3) and PLXND1 knockdowns in primary HUVEC used for cell collapse experiments.Western blots for GIPC1, GIPC2, PLXND1, and GAPDH (loading control) from TCLs of cells infected with the indicated shRNA lentiviral particles. Note the effective decrease of GIPC1-2 and PLXND1 levels. GIPC3 expression was absent under all the experimental conditions assayed. Hence, for brevity, the corresponding Western blots are not shown. This figure is related to Figure 7—figure supplement 3. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31050647), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Zebrafish Plexin D1 by Western Blot Efficient shRNA-mediated knockdown of GIPCs and CRISPR/Cas9-mediated knockout of PLXND1 in HUVEC/TERT2 cells.(A–H) Western blots for GIPC1-2, PLXND1, and GAPDH (loading control) from TCLs of stable cells demonstrating the effective decrease of GIPC1-2 and PLXND1 levels. (A–D) TCLs from non-targeting gRNA#1 cells (A, C) and PLXND1 gRNA KO#1 cells (B, D) infected with the indicated shRNAs and under the different SEMA3E treatments. (E–H) TCLs from non-targeting gRNA#2 cells (E, G) and PLXND1 gRNA KO#2 cells (F, H) infected with the indicated shRNAs and under the different SEMA3E treatments. This figure is related to Figure 7 and Figure 7—figure supplement 1. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31050647), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin D1
Plexin D1 is a type I transmembrane glycoprotein that is the prototype of the plexin D subfamily of semaphorin receptors (1, 2). Human Plexin D1 contains a 46 amino acid (aa) signal sequence, a 1225 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other plexins (1). The human Plexin D1 ECD shares 89% identity with mouse Plexin D1, and ~84-92% aa identity based on incomplete sequences of rat, bovine, porcine and canine Plexin D1. It contains a sema domain, two plexin-semaphorin-integrin (PSI) or Met-related sequence (MRS) cysteine-rich motifs, and three glycine/proline-rich IPT/TIG domains which are immunoglobulin-like domains found in plexins, transcription factors, and the scatter factor receptors Met and Ron (1, 2). Isoforms of 1787 and 1747 aa have been sequenced; these contain a 178 aa N-terminal deletion with or without a longer alternate C-terminus (3). Like other Sema/plexin interactions, Plexin D1 interacts with Sema3C or Sema4A via neuropilins. Interaction with Sema3E, however, is direct (4). Plexin D1/Sema3E interaction mediates vascular guidance during development or angiogenesis; deletion of either molecule results in similar, profound cardiac abnormalities (4, 5). Plexin D1 is also expressed in lymphocytes, osteoblasts, the neural crest and the central nervous system during development (2, 6). In the brain, the presence of neuropilin can change Plexin D1/Sema3E interaction from an attractive to a repulsive signal (7, 8). Plexin D1 directs migration of thymocytes to the thymic medulla, probably through repulsion of Sema3E (9). Endothelial cell Plexin D1 binding to Sema4A can oppose VEGF and suppresses tumor angiogenesis, and expression of Sema3E correlates inversely with tumor metastasis, indicating that Plexin D1 is anti-metastatic in the presence of its ligands (10, 11).
- Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
- Van Der Zwaag, B. et al. (2002) Dev. Dyn. 225:336.
- Entrez protein Accession # Q9Y4D7, EAW79239, EAW79240.
- Gu, C. et al. (2005) Science 307:265.
- Gitler, A.D. et al. (2004) Developmental Cell 7:107.
- Zhang, Y. et al. (2009) Dev. Biol. 325:82.
- Chauvet, S. et al. (2007) Neuron 56:807.
- Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
- Choi, Y.I. et al. (2008) Immunity 29:888.
- Toyofuku, T. et al. (2007) EMBO J. 26:1373.
- Roodink, I. et al. (2008) Am. J. Pathol. 173:1873.
Product Datasheets
Citation for Human Plexin D1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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GIPC proteins negatively modulate Plexind1 signaling during vascular development
Authors: Jorge Carretero-Ortega, Zinal Chhangawala, Shane Hunt, Carlos Narvaez, Javier Menéndez-González, Carl M Gay et al.
eLife
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
